XB-CLONE-3187635
Clone Name: IMAGE:7850813
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Gene:
klhl21
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389420 [+]
cttaaccactttaacttgagggcaagaggatttagcaggggataccagaaggtcatcttttgctgtgtttacagatgatcagccactgagggcctggatc
atggatacacccatcaacacttcccccagttcagcggggattccagcatctgaggcatccctgcctttctacgatgcctcccatgctctgcacttgttac
ggggcatcaatgagctcagggctgaacaaaaattctttgacatgacagtgagcgcagggggacaggatttcccttgccaccggactgtgctggctgcagc
cagcaactactttcgagccatgtttgctgggcggctacgggagagccacgttgaccgagttgaacttcatgatgtgactggccccatcctttccctgctg
cttgacttttgctacactgggagggtgacagtcacactggagaatgtggagcccctgctgcaggctgcagatcttttccagttcccatctgtaaaggaag
catgctgcgcctacctagaacagcaacttgatgtctcaaactgcctagagatccaggactttgcagaagcctatgcttgtcagggccttgcagagagtac
caaacggttcattttggctcacatggcacagctggcacaggcccatgaggtggagcgactcacctttaagcgaatgttggagtatatcagcgatgacagg
ctatgtgtggataaagaggaggctgccttccagatagccttgcaatgggtaaaggctgaccccaagcaccggcagtaccgctggccagagcttttccagt
accttcgattgccattcatacggcgcttctacctgctggcttatgtggaaagcgacccactggtataccgcagtccatactgcctacagctaatc
3' EST: DT389419 [+]gaacacagctgctgaccatacgggcccatgcagctgctctaggactgcagaaatactagggtaccttagcatcccttttgaagaatgttcctggtgtgac
attagccttacatgcactgtcaaacactgtgattacaaaccaaaacaaacaaaaaaaacttgtaactccttggaatttactggcatactgtgaaatgggg
gcaactgaggatatataaacattttttgtttattagaaaaatatatatattcttcctcttgtctatagaaatgataaagagcagctacatgtaacaatat
acatatattctatgtatgcgtacctatgggcaatgcatggacactgaaagtaatgatttgattgtactatgaagccaagagcactctcatacactcgtgt
aatgataaccgtgctgccaaagcatgagcttatcagtcacagagagagagtgtcaccgccccccactgtcactctgttcatatttaagtttcattagaaa
cccacttggcattcctatcgtcgatgtaattcattaaggttgtgattatgcaagtgctgtcgtcttctgttaaggttaattacgtttctctcgttgttga
cgttgtccaacggaaccccatctaaactgtactgagtttgtggcataaactgccggaatatactgacgctgccgtgccagaaagtcggctgagggaggcg
ccccacaaggctccacaatcttgtttcagggtcaaaggcttccacaacgtcagagagttcaaatgtgttgtcataaccaccagacacgtaaagcttcccc
cgagcgcagccaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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