XB-CLONE-3187587
Clone Name: IMAGE:7850817
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Gene:
ankrd9
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389452 [+]
agcttgaaagtggtagaagaagaaggaaaataatttaagaactatataaaatgaacaccaacggcaaagttgctaagaatatgctgtaattctataatat
actaaaacgtaactaaaaggtaaacaaccagtttaaagagaaagcctcagtaaaaataagactacctgcatgcattcatttgaggcaccttcccagcaaa
caggccaattaacatgttctttattcatagtgttaaaaaagaagtctgagaacaagtttctttatttctaaaagaattgcagatcagtagacttaaacct
ccattacacttattataaagcagattgtagttgtgtccaccaaaccagttctattggggatatttatttaagggtgacgtagagctgtctacaattcagc
agagaaatttcacagctctcagtttactgttgtcaggttttaagggacatagatattaaagagcaatctctcgcttgctcacagtgataaatgtgccctt
ttgtcttctccaggaatctattattctcagtaatgatttctaatgcatgattctcagcctgtaacgtttgctatagcaatgttatctacaaagatactga
ggaagaaacaagctcaaaataagaattgccgtgtaaaatacttcattcattatgaatgctcacatataagccaacatactatgcctcgggatctgcactg
ttcagaagaaggcagtggatgtacgacacacacacacacatatatatatatatatatatatatatatatatactgcagggcactttatactgatgtacat
atacaatcatgtctctgctacactgagatctctgcttacctcactttactggtactgtatttgtcaaaaatg
3' EST: DT389451 [+]cgtattgcaaatgtcttgtatttgcataacttacatttatcagtgctacaatggaaacagaaactaaatacacatcatttttgacaaatacagtaccagt
aaagtgaggtaagcagagatctcagtgtagcagagacatgattgtatatgtacatcagtataaagtgccctgcagtatatatatatatatatatatatat
atatatatgtgtgtgtgtgtgtcgtacatccactgccttcttctgaacagtgcagatcccgaggcatagtatgttggcttatatgtgagcattcataatg
aatgaagtattttacacggcaattcttattttgagcttgtttcttcctcagtatctttgtagataacattgctatagcaaacgttacaggctgagaatca
tgcattagaaatcattactgagaataatagattcctggagaagacaaaagggcacatttatcactgtgagcaagcgagagattgctctttaatatctatg
tcccttaaaacctgacaacagtaaactgagagctgtgaaatttctctgctgaattgtagacagctctacgtcacccttaaataaatatccccaatagaac
tggtttggtggacacaactacaatctgctttataataagtgtaatggaggtttaagtctactgatctgcaattcttttagaaataaagaaacttgttctc
agacttcttttttaacactatgaataaagaacatgttaattggcctgtttgctgggaaggtgcctcaaatgaatgcatgcaggtagtcttatttttactg
aggctttctctttaaactggttgtttaccttttagntacgttttagtatattatagaattacagcatattcttagcaactttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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