XB-CLONE-3187474
Clone Name: IMAGE:7850971
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Gene:
slc5a8
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389526 [+]
agagggtgactggagcctagaggggcacaaggcagggagctgtaggcgcagcacagccgtgttacaccgacaccatgtccggagacgagatgattttcga
cccaactatgaccagaaagaaaaagaagaagaagaagcctttcatgctggatgaagatggggctgagccacaggcagaagagacgcaggtaccggagact
aaagaagtggaaccagaaccaccagaagataaggattttgatgtcgatgatgaggcctttaaaaagaaagtcgatgcccctgatgacctggatgatttga
atttcttgaaccagaagaagaaaaagaagaaaacaaaaaagtttgacttggatgaagcagaggaaggggttaagaatcttaaaattgagggggagattca
ggaggcatctgaatcccaggaggatgatttggatttcatgctgagcaagaaaaagaaaaagaaaaatgtgaaattcccagaggaggacgataccctggat
agagatgaagccttcgaagaagaagacaataagaaagatgatggaatctcgttcagctcccagctgggtccggcctgggccggcacagaaagggactata
catacgacgagctgttaaacagagtgtttaatatcatgcgagagaagaatcccgacatggtggctggggagaagaggaagtttgtcatgaagcctcctca
ggtcgtcagagtagggacaaagaaaacatcatttgtcaactttaccgatatctgcaaactgttacatcgtcagccaaaacacttgctggccttcttgtta
gctgaattgngtacaagtggctctatagatggtaacaaccagttagtcatcaaaggcagattccagcagaagcaaatagaaaacgttctaagaagatata
tcaaggaatatgtgacttgtcacacctgccg
3' EST: DT389525 [+]ttttgtgaatcatgtaataactttaatgtatttttgcattttacagaggtttggttaaaaatgcatacactaccttcccacttcccctcaggcagctgga
aaaattgttttccgagagcaatgcgagtatatggaataatcggctccattgtctgctttagaatctctttgcgcgggcaaagatttaaaaaaaacaaaaa
aaaattagccaatgtgttttaggcctcttctcacagcattccttataatgccagggtactgcgccccctgtcagcaagtccctgcaatatatcctcgcac
caggctggcaaaatcccaaatggtttggaagccagttagttggctttggcacggagctgtgctctcttgcccgtgacggcctggaatccggttttgatgc
tggccacggaacaccgcgagtggcaggtttcgcactgcaggaaatataatcgggtgtccttctgcaagatggtgtctggcgatcggcaggtgtgacaagt
cacatattccttgatatatcttcttagaacgttttctatttgcttctgctggaatctgcctttgatgactaactggttgttaccatctatagagccactt
gtacccaattcagctaacaagaaggccagcaagtgttttggctgacgatgtaacagtttgcagatatcggtaaagttgacaaatgatgttttctttgtcc
ctactctgacgacctgaggaggcttcatgacaaacttcctcttctccccagccaccatgtcgggattcttctctcgcatgatattaaacactctgtttaa
cagctcgtcgtatgtatagtccctttctgtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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