XB-CLONE-3187430
Clone Name: IMAGE:7850927
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389555 [+]
agaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccgatatacgtgacaaacaaacacaggggggtgtcggctctgcaaggtcag
actcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctattaaactacactgggggggcaattaaatgtaaagaaaccaaggggggc
agtttatatgtggcattaacagcataactgactagttccccactagtaatactattatatagaacccccacacattctaccacattctgcctcctgcggt
ggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacgggggcagaataacccaggcagtcatgataaataccctgagttacaca
caaacatagcaggagctgccccgttcctttctatctgccccggggaattcccaagcgatcggaccggcaccaaatgttcatttcttttttgccagaattt
tcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgtggggactggtaccccgtctcgtctgctgaataaggagacactgacgc
ccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagccagttctactttgtagtagccaggggggggtccaaccaacccctttcat
ccagttcagatctgatttgtacttaatatcgctctgcaggctgtgggcttgcttggcgtgtgtcatggctagctgctgggggtcacacgtgtagcggtgc
aagtgctgccggtagtggatgtcgctggcttgttcctggctctttttggctt
3' EST: DT389554 [+]cgcaggatccccccacatcgaggcagtgaagaaggccggggagctaataagtgagagtaagtaccggcagaacccgggctccatggctttcacctctgtg
caggactcccccgacctgctccatgccaagagcagttacctgcagtgcaatgagagactgtacagatctggggactctggatcgttgcacagatacaccc
tgcctcctgatcaccccgactttatccgagctcgtatcaacgcctcccagatcagcgataaagcctataaaacctcgtgggagcagtcgcgggccgctgg
gtacgacctgagactcgacgccattcccttccaaaccgccaaggtgtccagggagatcgccagcgatttcaagtacaaagaagccttcatcagggagaag
gggcggcagattgggttccgcagcatcagcgacgaccccaacgcccaacacgtcctgagggtcagcaaactgcagagcgacagggagtacaagaagctgt
cggaggagagtcgcccccgagtcagaaccaacctggaccaaccgggtttcatccaagccaaaaagagccaggaacaagccagcgacatccactaccggca
gcacttgcaccgctacacgtgtgacccccagcagctagccatgacacacgccaagcaagcccacagcctgcagagcgatattaagtacaaatcagatctg
aactggatgaaaggggttggttggacccccccctggctactacaaagtagaactggctcgccgggcggcccaactgtcatacggagatggcacagagtca
gaccgggcgtcagtgtctccttattcagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: