XB-CLONE-3187404
Clone Name: IMAGE:7850977
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Gene:
gstm1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389572 [+]
tttggaaggaggacagaagctcagtaatagtgttggggttcagaatgatactaggatactgggatatcagagggttggctcattctatccgtctcctgct
tgaatacacaggcacacaatatgaagaaaaaaaatatataacgggggatgcccccaattatgacaagactcagtggctggatgaaaaagagaagctggga
ctggacttccccaatttaccatacctcttggatggtgatgtgaagctttcacaaagtaatgcaatccttcgctatattgcacgcaagcacggactttgtg
gagagtcagaaaaagaaaagatgtatgttgatctaatagaaaatcaaaccatggattttcggatgggtcttgttgtaattgcttacaatcctcaatttga
aacactgaaggggccatatttagaaaagctgcctattgctttgaaaagattctcttgctttctaggagacagatcctggtttgcaggcgataagatcact
tttgtcgattttgtggtgtatgatgtcctggaccagcatcagattctagaaccaacttgtctgcagaattttaagaacttgcaggactttctaaaacgat
ttgaggctctccctgctattgatgcttacatgaagtcctcacgctttatgaagatgcccctaaacaaccgcatggcgtcctggggcaacaaatagggcac
tatacagatggaagacctcttctgttttacaccagaacatgttcacaagtcattatttcagcgttttattttttctgtaatcatcagatcaccttgtctc
tcccctccctggtgtcattatggttggtaacttgatttacaataaagcccac
3' EST: DT389571 [+]ttcctctgttggctttattgtaaatcaagttaccaaccataatgacaccagggaggggagagacaaggtgatctgatgattacagaaaaaataaaacgct
gaaataatgacttgtgaacatgttctggtgtaaaacagaagaggtcttccatctgtatagtgccctatttgttgccccaggacgccatgcggttgtttag
gggcatcttcataaagcgtgaggacttcatgtaagcatcaatagcagggagagcctcaaatcgttttagaaagtcctgcaagttcttaaaattctgcaga
caagttggttctagaatctgatgctggtccaggacatcatacaccacaaaatcgacaaaagtgatcttatcgcctgcaaaccaggatctgtctcctagaa
agcaagagaatcttttcaaagcaataggcagcttttctaaatatggccccttcagtgtttcaaattgaggattgtaagcaattacaacaagacccatccg
aaaatccatggtttgattttctattagatcaacatacatcttttctttttctgactctccacaaagtccgtgcttgcgtgcaatatagcgaaggattgca
ttactttgtgaaagcttcacatcaccatccaagaggtatggtaaattggggaagtccagtcccagcttctctttttcatccagccactgagtcttgtcat
aattgggggcatcccccgttatatattttttttcttcatattgtgtgcctgtgtattcaagcaggagacggatagaatgagccaaccctctgatatccca
gtatcctagtatcattctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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