XB-CLONE-3187383
Clone Name: IMAGE:7850931
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Gene:
ddx3x
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389586 [+]
aaatcagccaggcctgtcacagaaccacacactgctgtggtcagccattctgctatatcttcaggacaggcatatgtcaatttatagatgatacctccta
aaaactatttaaagtttcagattgttaaacgtttcctgtaaagatcggcccttatttggcaaactcacaagttactgatctgccttaacattgttaaagg
aattgccgtattcacttgcattctctgaaaacagtgcttaacatgtatgattgacattcatacaccttagtgattggaatggcttaataaagagacttct
ggtttgagtaccttttccaggatagagtgagcaaatcagaacttaacttggtacagtgttccacattagaaatgatttcttccgatatgcttcaaggtgt
tttgcctcaaattggcaagtcaacagaagccctaatgcagggttactgcacaagtttttccttgtaatattaatgcatggatgaagagattcctgttttt
gagttgtatcacacctgtgtgccaaggtttgatttcgcccaagttcaataaatttattttgtctcaacaatttataccttgagcaatactgagccaacag
gacatcaaaataaaaagttgtctaaagttaaaggcacagtacattaacaaacaaattatcctcagtcaaaataaatgcagttgttgggagggggtggatc
tgggttttaatccaacactacagcaatgaaatcttcaacaccttcagagtagggcttgtttctattgtttgctttctttgtggcccagagctcccttgaa
aagaaaatcagtggagaatcacagctggtaatgtacaagttcttgaagctacacaaggtatagtctggc
3' EST: DT389585 [+]aaacaagctaatatggaaaccacatgtaacttagccagactataccttgtgtagcttcaagaacttgtacattaccagctgtgattctccactgaatttt
cttttcaagggagctctgggccacaaagaaagcaaacaatagaaacaagccctactctgaaggtgttgaagatttcattgctgtagtgttggattaaaac
ccagatccaccccctcccaacaactgcatttattttgactgaggataatttgtttgttaatgtactgtgcctttaactttagacaactttttattttgat
gtcctgttggctcagtattgctcaaggtataaattgttgagacaaaataaatttattgaacttgggcgaaatcaaaccttggcacacaggtgtgatacaa
ctcaaaaacaggaatctcttcatccatgcattaatattacaaggaaaaacttgtgcagtaaccctgcattagggcttctgttgacttgccaatttgaggc
aaaacaccttgaagcatatcggaagaaatcatttctaatgtggaacactgtaccaagttaagttctgatttgctcactctatcctggaaaaggtactcaa
accagaagtctctttattaagccattccaatcactaaggtgtatgaatgtcaatcatacatgttaagcactgttttcagagaatgcaagtgaatacggca
attcctttaacaatgttaaggcagatcagtaacttgtgagtttgccaaataagggccgatctttacaggaaacgtttaacaatctgaaactttaaatagt
ttttaggaggtatcatctataaattgacatatgcctgtcctgaagatatagcagaatggctgaccacagcagtgtgtgggtctgtgacaggcctggctga
ttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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