XB-CLONE-3187317
Clone Name: IMAGE:7850889
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389630 [+]
atgcctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccg
atgagtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccga
cgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaa
aaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagc
gctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaa
tgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaag
aggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatgg
ttacccccgggcacgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggagg
aatcaccttcctgtc
3' EST: DT389629 [+]atagggcgagaggccagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacg
tagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgca
gtctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcac
taggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcct
ctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgccccc
ccaggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacg
gtggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacct
cctgaggggggtacttcttggtgcaggcgtgcccggnggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagcctt
gtacactgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatcaggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: