XB-CLONE-3187190
Clone Name: IMAGE:7850805
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Gene:
pcmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389713 [+]
ttttttgtgattttgctttgtattttatttggcaaagcagagctgcataaacagttcggtaacatgaacataccttacacagcaagtgtttgtttcttat
atggagtagtgacctataaactgcatcaatgggagggtgcttaaacataatgcaggttgccaattattatttggcctgaaagaggatggatttttaaaag
ccaagtctgaacatctgaagaatttatgatgattagagccttaactgtagactaaagtgtaatgtacttcctggatgcaaattaaattaggacaaagaca
ttggccagtagtaaggcgaagctgctgactgcatccacaacatgcttttattctaagacaccagttcaaataaaggaagctcatttctatatgtttagca
gcatttagcagaaacttgatagtatgcatatgtgaagcatttgatcaactttaaatgtagcaacaatgctgtacactgcagttttcatgtatctggaaat
taaaacagaatgcgtgcaattttaattaaatagctgacaagctggttgcttgaattaaattggtgaaacttttttttttttaaatctaaaataaacttaa
atatgcctttccccaaattattctgcaacctattttagtaaaaaaaaaaaaaaaaaagaaagccagaaaacaaacattccacaccgcttttaacagggta
ataaagtccaaaacaaactgcactgctgatgattctgaagtaaaagcttttgagaaggcagttaagcactgcattgcaatactttgtctgaatcatggag
ctgttacaattcatcccttttatgtgtttgggaagaggaaatagtggaac
3' EST: DT389712 [+]tcgtgtgctgtgtgcagtcatggcctggaagtcagggggagccagccactcggagctggtcaacaacctgcgcaaaaatggaattatcaaatcagataga
gtgttcgaagttatgcttgaaacagatcgccgacattacgcaaaatgcaatccttacatggactctccacagtcaataggttatcaagcaaccattagtg
caccacatatgcatgcatatgcactagaacttcttcatgatcagttacatgaaggtgccaaagctcttgatgtaggatctggaagtggaattcttactgc
atgtttttctagaatggttggcccaaagggaaaagtagtagggattgaccatatcaaagaattagtagatgattctataaataatgtaaaaaaagatgac
acaaccttgttgtcttcaggccgagtaaagctattagttggagatggtagaatgggttacccagaagaagcaccttatgatgctattcatgtaggagcag
ctgcacctgttgtaccacaagctctgatagatcaacttaagccaggaggaagattaatcttacccgttggtccagctggtggaaaccagatgttggaaca
atatgataagctagacgatggaagtgtcaaaatgaagcctttgatgggggtgatctacgtgcctttaacagacaaagaaaagcagtggtccaggtggaag
tgaatttctgttccactatttcctcttccaaacacataaaagggatgaattgtaacagctccatgattcagacaaagtattgcaatgcagtgcttaactg
ccttctcaaaagcttttacttcagaatcatcagcagtgcagtttgttttggactttattaccctgttaaaagcggtgtggaatgtttgttttctggcttt
ctttttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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