XB-CLONE-3187163
Clone Name: IMAGE:7850855
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389731 [+]
cgaggacacactgaccatggaagcgattccattccagtcggccaagtcttccgcagacatcgccagtgattataaatacaagcacaactatgtgacggag
agagggaagcacatcgggtcgcggagcgttctggacgacccccagatgctgcactgcctgcgcgtggccaagatacagagcgaccagttgtacaggaagg
actcccaggacctgcggtcccaataccatctgcccatggacatgatgaatctggcgcaggccaagagggcccaggcgctggccagtgagcaggactacag
gctgaggctccatcactacacggccgactcgcaggacatgaggcttcagtgtgccaagcgggcaaatgagctgcagagcgagaggcgctacaagtcggat
ttactgcacacgcggggagtgggctgggaggccgcaggatccccccacatcgaggcagtgaagaaggccggggagctaataagtgagagtaagtaccggc
agaacccgggctccatggctttcacctctgtgcaggactcccccgacctgctccatgccaagagcagttacctgcagtgcaatgagagactgtacagatc
tggggactctggatcgttgcacagatacaccctgcctcctgatcaccccgactttatccgagctcgtatcaacgcctcccagatcagcgataaagcctat
aaaacctcgtgggagcagtcgcgggccgctgggtacgacctgagactcgacgccattcccttccaaccgccaaggtgtccagggagatcgccagcgattt
caagtacaaaagagccttcatcagggagaaggggcggcagattgggttccgcagcatcagcgacgaccccaacgcccaacacg
3' EST: DT389730 [+]gagaggttcaatggagtcgcgtttattccgtacaaacgattctacaaagaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccga
tatacgtgacaaacaaacacaggggggtgtcggctctgcaaggtcagactcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctat
taaactacactgggggggcaattaaatgtaaagaaaccaaggggggcagtttatatgtggcattaacagcataactgactagttccccactagtaatact
attatatagaacccccacacattctaccacattctgcctcctgcggtggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacg
ggggcagaataacccaggcagtcatgataaataccctgagttacacacaaacatagcaggagctgccccgttcctttctatctgccccggggaattccca
agcgatcggaccggcaccaaatgttcatttcttttttgccagaattttcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgt
ggggactggtaccccgtctcgtctgctgaataaggagacactgacgcccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagcca
gttctactttgtagtagccagggggggtccaaccaacccctttcatccagttcagatctgatttgtacttaatatcgctctgcaggctgtgggcttgctt
ggcgtgtgtcatggctagctgctgggggtcacacgtgtagcggtgcaagtgctgccggtagtggatgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: