XB-CLONE-3187044
Clone Name: IMAGE:7850962
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Gene:
abcf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389810 [+]
acggactggggttcacgcccgccatgcagcgcaaaaagctcaaggatttcagcgggggctggaggatgcgtgtggcccttgccagggccctcttcattcg
cccattcatgctgctcctggacgaaccgaccaatcaccttgatctggaggcctgcgtgtggcttgaggaggagctaaagtcgttccggcggatcctggtt
cttatctctcactcacaagatttcctcaatggcgtctgtaccaacatcattcacttgcacaaccgcaaactgaagtactacacgggtaattatgatcagt
atgtgaagacgcgcctggagctggaggagaatcagatgaagaggtttaattgggaacaggaccaaatcgcacacatgaagaattacattgcccggtttgg
gcacggcagcgccaagttggcccgtcaggcgcagagcaaagagaaaaccctgcagaaaatgatgtcgtccggcctaacggagcgggtcattaacgacaag
actctgtcgttctttttcccttcctgtgggaaaatccctcccccggtcattatggtgcagaacgtcagcttccgctacaccgccgacgggccgcttatct
acaagaacctggaatttgggattgacctcgacacccgagtggccctcgtgggccctaacggagctgggaaatccactctgctgaagctcctgactgggga
gctgctgccatcagacggaatgatccggaaacattctcatgttaaaattgggcggtaccatcagcacctgacggagcagctggacctggacctgtcgccc
ctggaatatatgatgaagtgttaccccgagatcaagg
3' EST: DT389809 [+]gtagggattctctttattgtctcagggtaagttacagataaataattggcttataaaagaaaaggagtaagtgtgactgagtgtgagtgagcttgaggct
cgagtaggagagccaccctccattccttcaggctccagtgaagtcgcacagagttacagtcctgagctgcagttcagtctcggagccaccagggggtaag
gggggccttgggcgcatagagctcgggctccaacaaggggccgactgggaaccgggactccctgtctgttggttacaaataggaataagccccaaactgg
ttgcttaggagaccgttgcttcttagttgcccctgcactgggacagctttgcttttcagttgcccttaaattggaagatccttgtgtctcagttgcccct
acactggggttcatcgtctccgagccgggatttaaggtgctgtttgtaggacaggatatcccccgcccacttggttacggcctgtttctcacacacccag
atctcctgagccacctgctgtataagccggaagtcgtggctcacaagcatcatccccccttcaaactcattgatggcgtctgccagggcatctatcgtct
caatgtccaagtgatttgtgggttcatccaagaagagcatgtgggggttctgccaggccagccacgcaaagcacacgcggcacttctgcccgtcagacag
gttacggatgggactgacctgctgtttgccagtcagcccatatctaccaatgatcttcctcatctcctccttctccttgatctcggggtaacacttcatc
atatattc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: