XB-CLONE-3187015
Clone Name: IMAGE:7851108
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Gene:
rell1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389829 [+]
agaatgggtcgggagagcgctcctgagcgtaacgtttcacctctagtgaccctcaaaactcgaattcggacgttgaaaatacaactgtcgacaacaaccc
caccaacccgcaatatgtagcctttgtgttggtgcccatttttttcataatgggtcttatcggagtgttcatctgccatatgcttaagaagaaggggtat
cgctgtactactgaggctgagcctccagtggatgagaaaatcataggagaaaaaatcgaaatgcatgaaagcataggggatacgaatactgacaccgttg
gtcatattgtcaactacattatgaagaacgaagctaatgctgatgttctaaaggccatggtggcagacaactcagtcattggggagacaagtgtatttga
tcccgagagtcccactactccaaacacaccgaccagtcccaccagtcctgacactcctatgagtcctatctccccaactgaaacgccgtcgaagcattcc
tgccatgggaaccattttcacaccgttgggggagtggctgaaaaaatgtgtgcactcgctgcaccaataaaaggtggcacttgataaggtcaccccaaaa
acataaagagccacgaagaagccaccaaggagcagtgactgttttgtctgtgggaaggttcagggtcaccaaagtggaacccaagtcaaaagaaagaaag
aggctaatggctgaaaggacggaaggtactaatggtgaggtccctgccactccagttaaagtggataccaggcagagaagtggcacagattccgagcctc
anagtcctgttgaaacctgaggagttaagtgcaagaagctgtg
3' EST: DT389828 [+]gttgtcactcttattttttattgtatatttgtctttttgaggcctactcctatttctgttcctttttgcagcaaagtgaagtggaatgggggggcagtga
agcagaggctgggggtgggcccctggtaccgcagaggctgggggggtgggcccccggtaccccagttcgatagtgttcctactgatcgaatcaatggtgc
aaagagccagaacaatcagaacagcagggagtgaagagcagcggtggctccagatacagtgaatcatggcccggagaaatggaaacctgaatgggcctct
aagccgcagagaagagtttgtcacatcgagcagatagttggtgaagaacaatatggttactgaaacggcacccatggctggcactttgggggacaagatc
tgtcagtctgttggttggtccttctgagaatcaccgataccctcagctgacaaacagaagtgttatacagcgtggctttatttacagcagccttcaccct
attctgagcaatggattgcactgtggccagggggtcccaggcacctgttactatgggggaagcccccagcacctataacattagccctcgcacaaacagg
cacagtcaggacacctgctgggaggtgaaatacatatatatatatatatatatatataaccatatctggcatacagggccagtctacatatacacatgtt
aaaagttcaacagaagaaaaattccactaaaaatacaaaatataaaggaaggcagttcttgttgtttgtatagaatggaagatccatactttcacataaa
gcccgaaccagtgggcagctcctaggtatatctgccgctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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