XB-CLONE-3186976
Clone Name: IMAGE:7851064
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Gene:
csrp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389855 [+]
aaagtaaaaaaaagagtaactttatttctgtataaaaatataaatacaaaacttccatacaatcggaagagcacatgaaaatatgcacattattaggcat
ttattattatatgtacattaataaatgaaataagacattcgggaggaatgaagaagcactttattataaagcataatgaattctcgatacagaattggtc
atgtgatcacattgctccaaacccgagcgagtttattggtttacaggtcggtttcactgaaactaacagattttgggaaggatcacaccgagaggcggca
ccgtgtggcccagtaatggggaatagaaaacccgttgcagactgataataacaacaagagaaagagcgtaagggcatttacagcggcggctgatttccca
ggaatgacagcggaatccggcctggtacctataggaaaatagtgcattaatggagagaacaagtggcgttagtgctgaatctgtaaaaaagaagaggaat
gaagagattagtgcagcccggcagcggaacgcgttttgctgcctctttcctacctgcaaataaaatcacgttaagggcaggagctcctaaagggaaacag
gttaggcttgtggggagagaagctggaaagaaagaggttaaacagcattggatctgacctcctagaaagagaaagccattaagaaaaaggctatggcagc
catggttccccactgctgctccccttcattaagaagcaccctatcactaatgngaacccggtgctgctctaggctctccctacggatccccgtgccgctg
cccaggcactcacatatgtcccactagccattcacgcttagntggttagactggatg
3' EST: DT389854 [+]aatatgcccctgagtgctgctttacttgttattggtaaccccccccccaataatccctaaatatgtctgaccctccctcattatttgccccagccctccc
ctgggcattccctgcatgtgggcagagttgcacacaaggggggcagggcaagtgccattttcctgtgcaagaggggtgtaggattgtaccaaaggcgccg
ggggtcacttccctgtgctactatgctttaagataaattgccatttgagcaaccttacaagtattgagttaatgtgtatttttaattttcagcaaagtga
ggagctatgtgctgtgctaccaggcccagagcatggcgtttcagggcatggacataggggtatgagcataggcaggggcggggccagcagcacttggtag
gtacatactatgtgtagggggtctctgcctgtgagacataagagtgctcttttgggtaccattatgggcagccaggatccttcagggctccagcacttat
ttatttccattagattcctagttttgcacattgcagattctatgcactgatctttggggtgcctatagggggcgctcattcacagtttaaatatagtcac
actgatttaccattaaatcctatggatttgtgtttttatggcattggccttgctccgtgggaaggttcttactggattccatgttgggatcttattgtta
tattgtcacattgccggggaggatggtggtatagctgtcactctgcccagctgtgcgcttcctaacggcactttatggcacatttctgtcactagacaca
gggctactagtaatggctagtgtactgactgccccatgtacggcctggtccagtcccaccactcatccagtctaaccactaagcgtgaatggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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