XB-CLONE-3186923
Clone Name: IMAGE:7850782
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Gene:
MGC75753
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389890 [+]
gagtgagagggctagctctggcctgcttcccccccttcagcgaaacactcgggggtagaaagttgaggactctggctgccggagatcacaaaggaatcgc
gtacgattctgtcctgcctatacgatcgaagagagagacccgctgctgttgatgtttatatagctgtgaggattcaaccttatatgccacaatgatgact
gagatctcaacagctgctgcgtttatcacaagtttgctgaaaacaaccggggtcatagaggagaggcagctgcagcacttctgccaggtcctacaggagg
ccatgaaggatcattataaacaccactggtttccgcagaaccctttcaaaggatcagggtaccgttgcttgagaattaaccacaaaatggaccccttgat
aggacaggcagcaggtcacattgggctcagccaccagagactgttccagcttctccccagcgagctgactctttgggttgacccttttgaagtctcctat
cgcattggtgaggatgggtccatctgtgtattgtatgaaggagccgcagcagggggcaagcctattgaaaaccatgcaagctgcaaggatgcacttagaa
ttggccggaatagcccctccaaaaattacagcataatgacagtttctagttaaacagctgaaaaataaatcaggaattgtacaattacgtatgtgtccct
cttctgccttgatttgcgttaatgtttcatcttcattccataaacattaaaactgtaatactgccaaaaatgtaccgtcttgttgcagaaggtgaatgta
gaataccatctggggcaaaaagctcttattgtcatttctgtctttcttttatgacattttctcgctccggtctctgaaattgtacattgtaaaatgaaat
c
3' EST: DT389889 [+]tttttttttttttaaaatagtttttctttttattaaaaagagccaatggttataccaaaatataaattctctcttgactcatgatttcattttacaatgt
acaatttcagagaccggagcgagaaaatgtcataaaagaaagacagaaatgacaataagagctttttgccccagatggtattctacattcaccttctgca
acaagacggtacatttttggcagtattacagttttaatgtttatggaatgaagatgaaacattaacgcaaatcaaggcagaagagggacacatacgtaat
tgtacaattcctgatttatttttcagctgtttaactagaaactgtcattatgctgtaatttttggaggggctattccggccaattctaagtgcatccttg
cagcttgcatggttttcaataggcttgccccctgctgcggctccttcatacaatacacagatggacccatcctcaccaatgcgataggagacttcaaaag
ggtcaacccaaagagtcagctcgctggggagaagctggaacagtctctggtggctgagcccaatgtgacctgctgcctgtcctatcaaggggtccatttt
gtggttaattctcaagcaacggtaccctgatcctttgaaagggttctgcggaaaccagtggtgtttataatgatccttcatggcctcctgtaggacctgg
cagaagtgctgcagctgcctctcctctatgaccccggttgttttcagcaaacttgtgataaacgcagcagctgttgagatctcagtcatcattgtggcat
ataaggttgaatcctcacagctatataaacatcaacagcagcgggtctctctcttcgatcgtataggcaggacagaatcgtacgcgattcctttgtgatc
t
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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