XB-CLONE-3186841
Clone Name: IMAGE:7850980
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Gene:
slc35f6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389944 [+]
acccacatctgccctcttgtgcccccagatacccccacatgtcccccatcttcccccatgtgcccccatatttccccatatgctgcatttagcgctggtc
actcagtgttcatgtgactgcactttggctcagcgcggcctgtgattggctgttagttatttccttgtgtctttggacaaatcacttaatctccttgtgt
cccaggttgaaggattagactgtaagctccatggggaaaaggccaggtggggatgatgcacagttaccccaatgtcccgtgcagggaatgggtcagtacc
gaccagacgccccgatgcacaaaataataataattcataggacggacagagatgaacccaaatgtatcagaaccgagaggcagtactgagatgatattaa
tattatataacattatatatatatataagtgcctgctgtgtaactgcccgagggccacacgtcccccctgggccactttcctgtgtagcaaataagggaa
ttctgggccctggttctgatactgctgataaagggaaactgttctatgggaacggaccggctggtaccactgggccctggttctgatactgctgataaag
ggaaactgttctatgggaacggaccggctggtaccactgggccctggttctgatactgctgataaagggaaactgttctatgggaacggaccggctggta
ccactgggccctggttctgatactgctgataaagggaaactgttctatgggaacggaccggctggtaccactgggccctggttctgatactgctgataaa
gggaaactgttctatgggaacggaccggctggtaccactgggccctggtgctggttccgctcctgt
3' EST: DT389943 [+]aacaaatatctgtaaactttatttcgtgtttatttggctacaggagcggaaccagcaccagggcccagtggtaccagccggtccgttcccatagaacagt
ttccctttatcagcagtatcagaaccagggcccagtggtaccagccggtccgttcccatagaacagtttccctttatcagcagtatcagaaccagggccc
agtggtaccagccggtccgttcccatagaacagtttccctttatcagcagtatcagaaccagggcccagtggtaccagccggtccgttcccatagaacag
tttccctttatcagcagtatcagaaccagggcccagtggtaccagccggtccgttcccatagaacagtttccctttatcagcagtatcagaaccagggcc
cagaattcccttatttgctacacaggaaagtggcccaggggggacgtgtggccctcgggcagttacacagcaggcacttatatatatatataatgttata
taatattaatatcatctcagtactgcctctcggttctgatacatttgggttcatctctgtccgtcctatgaattattattattttgtgcatcggggcgtc
tggtcggtactgacccattccctgcacgggacattggggtaactgtgcatcatccccacctggccttttccccatggagcttacagtctaatccttcaac
ctgggacacaaggagattaagtgatttgtccaaagacacaaggaaataactaacagccaatcacaggccgcgctgagccaaagtgcagtcacatgaacac
tgagtgaccagcgctaaatgcagcatatggggaaatatgggggcacatgggggaagatgggggacatgtgggggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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