XB-CLONE-3186662
Clone Name: IMAGE:7850900
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Gene:
myl4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390063 [+]
cagaaatcagcagcaacttcattcccgtgcaccatgccacccaaaaagccagaacccaaaaaggaggcaccaaaacctgccgcagcccctccacctgcag
caccagagcccccaaaggaagtcccatttgacccaaagagcgtcacagttgagttcagtgctgaccaaattgaagaatttaaagaggccttccagctgtt
tgaccgcaccccaaaaggggagttgaaaatcacatatggtcagtgtggggatgtgctgagagctctaggacaaaatccgaccaatgcagaggtgatgaag
gttctggggagacccaagccagaagaaatgaacaccaaactcttggactttgaaactttccttccgatgctccagcatatctccaaaaacaaggatcagg
gttcttttgaagactttgtagaagggctacgtgtcttcgataaagagagtaacggcacagtaatgggtgctgaacttcgccacgtgcttgccaccctggg
cgagaaactgcaagagaacgaggtagaacagctgctggctggacaggaagatgccaatggatgcatcaactatgaagcattcatcaagcatataatgtcc
ggataaaacctgtgaatgtgacaactgcttgatcctccaatcgtctgtccttcagagtctgacccttttctgaagtgcccctaggtgtccctgaaaggaa
gaaacctaaaagaagatgaagctgtgctcttcagaaatgtcctgctcagttgtaaattatttgggatattttttttcccactgacttttcaccaaataaa
ttttaccaaactaaaaaaaa
3' EST: DT390062 [+]tttttttagtttggtaaatttatttggtgaaaagtcagtgggaaaaaaaatatcccaaataatttacaactgagcaggacatttctgaagagcacagctt
catcttcttttaggtttcttcctttcagggacacctaggggcacttcagaaaagggtcagactctgaaggacagacgattggaggatcaagcagttgtca
cattcacaggttttatccggacattatatgcttgatgaatgcttcatagttgatgcatccattggcatcttcctgtccagccagcagctgttctacctcg
ttctcttgcagtttctcgcccagggtggcaagcacgtggcgaagttcagcacccattactgtgccgttactctctttatcgaagacacgtagcccttcta
caaagtcttcaaaagaaccctgatccttgtttttggagatatgctggagcatcggaaggaaagtttcaaagtccaagagtttggtgttcatttcttctgg
cttgggtctccccagaaccttcatcacctctgcattggtcggattttgtcctagagctctcagcacatccccacactgaccatatgtgattttcaactcc
ccttttggggtgcggtcaaacagctggaaggcctctttaaattcttcaatttggtcagcactgaactcaactgtgacgctctttgggtcaaatgggactt
cctttgggggctctggtgctgcaggtggaggggctgcggcaggttttggtgcctcctttttgggttctggctttttgggtggcatggtgcacgggaatga
agttgctgctgatttctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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