XB-CLONE-3186600
Clone Name: IMAGE:7850858
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Gene:
zfp36
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390104 [+]
tcgattcaattcggccgaggattcacactctctatcagaacttgcgcaacctggatctttcagaagatcttgattctccaagagaagggaaattacttag
tactcaacggagacattcctgtacccctgacctcgatgatctgtacaggccctcaattgattcttggaactttgaccttccacgagccccattccgaagt
gaccgttcgataagcctgacagaaggaccacggttgacaattcctgctcctccacctggatttccacccctcaagactgctcttcctgcattacctgccc
cctctcctcgctacaaaacagaactgtgtcgcacattctctgaaacaggcatctgtaaatatggagctaagtgccagtttgcccatggcaagatagaact
tcgagagccaaaccgtcacccaaagtataagaccgagctatgccacaagttctacttgtatggagagtgtccctatggatcacgctgcaatttcatccac
caccccagtgagcaaggaacttcccagcacgtgttgcggcaaagccttagctattctggtgtgccaagcaagaggggttccccacccccacctggattac
cagaccctgctgccttctccagagcaccttcagtttctcctccaccatcctctgagctgctcttttctccaatccccactgaagcacgaagccatgtgtc
ttcactgagatcagccgattcttactcacattgctgctcatgtcgttgctccagagcaggaacaatcacccaggatctcctgtctacccacatgctcctg
cgctcaccttcatgcagctcactgccagagactgaatgctacagcagtgggtcagaatctccagtttt
3' EST: DT390103 [+]gtatgcaaatctctttatttcagattataaactcattgcaaatgctgacaaatagatattatttacaaaataataaaaaatgataaatatatttttcatt
tttaaaaataaatatgatataaatagattaatacacgttagaagaagcacactttaaggttaaagatcaaggtatgaattagtatcaggttttgctgata
aagtttctctgctccctacatttcctgtttgtgcctgattttaactccttgattgccagaaaggacctgtcagtgtaactgtgaggtaggacacacattg
ttaggaatgaccctttgctgctaggaagtactgcaatgcgtgcctaaccctgcaccatggatcatcaaagacagtagcatagtccaatgcaatggagttt
aaaaactagcaagcactgaatatgatctgtgtgtgtctacagtctgtctgatgggctgggtgctgtattataagcaaagcagcatgtgctgaagtttctt
atacacacaacactagaaatatcttttacacagaccaatatgtgcattatacaaagacagggtacatcgatatttatgtatatagatatatatctatatt
tatgtagagcagtatctatgttaaaggagccacgtatctttagtttgcacagagaaggttatgtctctattacacaaagagcagagtgcatctgtactat
tattcacatcagtgtgtgtccatgctatgtttagatgagagtttatctacagcaagtgcttggatattatatgtagagaggcacatgaagctgtgagctg
tgggcagaagctcaggtaaaataggctagctacaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: