XB-CLONE-3186588
Clone Name: IMAGE:7851098
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Gene:
KIAA1217
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390112 [+]
caacaatatttgaagaaggtgatgacgatctagaaaaacacatgtcagaggctaaaatagacgaagaagatgaagaagaggaagcagaagttccttcacc
ccaagaatcgaaaaaggagttaccaatggaacataccacaaaagcatcctcggagaatccaggtttggaaaacaagtacagattcaactatccaacttta
gccgaaatgaaaaagaccatcttcgaagacgccaatgccatacagacccatttcacagaggaagacaggtctgaagcttccgatgatcagcttggtcaaa
ggcaggatgtcaagaaaaaattcaagtttaaatttcccaaaaaacagctcgctgcgctgactcaagcaatccggacagggacaaaaactggtaagaaaac
attacaggttgtggtctacgaagaggaagaagaagcagatgggactataaaacaacacaaagaagcaaagagatttgaaattgcaagatcaagtagcaaa
gaggacaatgccaaatacggcgcagaagcagtgggacttgagacggacattcaggcgtcacggacagatgagattaaaagaaccacctacaagattctag
acagtcttgagcaaaccataaagcagcaggaaaacctgatgagcgaaatggcacccataataggggcagaggctttagaatctagagagaaatgtaaagt
ctccagtgacatcaagactggtgcagcggaaccagtggaagaaccaaaacctgttgtagagactccttcctccatccctactgcagcacgtaagggttcc
agtggaggagcacaaaccagcagaatgccaatcccaatgtctttctaaagtaggcaagcaaacgtggaca
3' EST: DT390111 [+]ttacacgttgataaaaatagaaatctttaattgtagccagcattaaagtaaaagaaaatagccttttttttgcagataaaatttcacgctagcgtttgtt
ttgttgttgttgcagacttcatagttaaaaaatatttaatataaaataatctctgtcctcgcaatctcgtttctctcgcctgttcatcacacatgcacac
atacatgaatatttacacacacacacgtgtattagttgtctatctccatgactctgcacacagataggaggtggttgaattacaatggcggcaggtttgg
ctgttagtcggtgactggtgcatttgagctgcagaaggtggagttgttggcacgcggcggcagacctagtaacagacatggtgggcctaagaggtctcct
tggcattcaggcctttgcttgcacttccgttctgtgacttgtagctggtgaagctaggtgtatggattgtgcggatgctcttcacaccttgagtcagtga
agtgggagagacagaagaagggggagaagaactagaagcagtagtagagggaagagctggtctgccatttgaggcctgcggtgagaatgaaagatggtga
cctttatatgtaggcgggggctgaagtttggatgagccattgtgagcaggacgtacaagggctgaagggttagcggtgcggctagcttgggggttagaat
tggcaacatgtgacttgacagagggattaggggggttagaggtctcgctagactgagcaggcacagagccacttttcccagtgttagaggaaaggcctgg
aatcttagaaacagataaagaaggggcagaagcccttacatccccaccagctttcttagtac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: