XB-CLONE-3186552
Clone Name: IMAGE:7851341
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Gene:
ppp1r1a
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390136 [+]
atccagttcactgtcccactgctggagccacatctggaccctgaagctgcagaacagatacggagaagaagaccaacaccagccacacttgttctgagca
gtgaccagtcatctccagagatagatgaggaacgtgtccccaaccctttacaaaagtcttctttatccatgtccccacatcagaggaaaaaaatgtcccg
tacaacacccaccatgaaagaactacaactattagcagaacatcatctgtgcaagcagggatcagaggaggagaagatcccccatcttcagaacaacctg
gatgacaggcctgacttgggatgctgttgccatggtaacacagcctccactcaagagtcgcatataccatgtagctgttttacccaggatggacagcaag
agaataattccctgggaaggagacatgctagtcaggaggattcattagacagtcttgtgtcagatggaaatacgcaaacatgtgagccaaaaaaacagag
cagccgcatagctttcatagaagacaaataggtctgaaaaagcaaaaggatttcttattgagtataaagggagagaggagccaaaacccagcacagtatc
gtgggcttacagcacataaaggaccgacagctccctccttttaaaaatgatttttccttacctctctgctactgagaatctggagtctgcactgagttct
gagcaataaactgcaggcttctctggaagcaaagaagttcaagaaaacattgctttgtgtttgatatcttgcctctgtactgtatgtcttctaccttttc
aagggaagtgtgtatctgtatgcagaaagccaaactggccgctgctatgccaatcattgttagcccttattagccctcaggatgagtc
3' EST: DT390135 [+]ttttttaaaagggcttcctccattttattaagaatttgacattataacgaatcgcatctggcaaatacatgttcccaatgcaggtgctgcagttgtggag
cagtgtggggatgtggcacgtttagtgatctttatttaaggtattgcaggtgagctttctgtaagcattccttctatagatgtagctgctaggtacagtg
tacattaatctacagtctttcttgggcctaggtattttttgcacacacaaagttattttttcatttaggtaaaacccaaaaagcacaaatgattttattc
tacccacatgttatatcctcccgactcatcctgagggctaataagggctaacaatgattggcatagcagcggccagtttggctttctgcatacagataca
cacttcccttgaaaaggtagaagacatacagtacagaggcaagatatcaaacacaaagcaatgttttcttgaacttctttgcttccagagaagcctgcag
tttattgctcagaactcagtgcagactccagattctcagtagcagagaggtaaggaaaaatcatttttaaaaggagggagctgtcggtcctttatgtgct
gtaagcccacgatactgtgctgggttttggctcctctctccctttatactcaataagaaatccttttgctttttcagacctatttgtcttctatgaaagc
tatgcggctgctctgtttttttggctcacatgtttgcgtatttccatctgacacaagactgtctaatgaatcctcctgactagcatgtctccttcccagg
ggattattctcttgctgtccatcctggggtaaacagctacatggtatatgcgactcttgagtggaggctgtgttaccatggcaacagcatcccaagtcag
gcctgtcatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: