XB-CLONE-3186285
Clone Name: IMAGE:7851269
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Gene:
nedd4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390313 [+]
ggccagagaatggttcttcttgatttctaaagaaatgttcaatccttattatggtctgtttgaatattctgccacggacaactatactttacaaattaat
cctaattctggtctgtgtaatgaagatcacctttcgtatttcaagtttataggccgcgtggctggaatggcagtataccatggaaagcttcttgatgcat
tttttattcgcccattttacaaaatgatgctccaaaaaccaattatattgtatgacatggagtctgtggacagtgaatattacaattccctccagtggat
actagaaaatgatccttcaggtttagatctgtgcttcactgttgatgaggaactttttggacagacacatcaacatgagttgaaggctggtggttcacaa
atcttagtcacaaacaagaacaaaaaagagtacatacatcttgttattcagtggaggtttatgaacagagttcaaaaacagatggctgcattcaaagagg
gtttctttgaactgatcccgcaagacctgataaaaatatttgatgaaaatgagctggagctgctgatgtgtggcttaggagatgtagatgttaacaactg
gcgagaacatacaaagtacaagaatggatactcacaaggacaccaagttatacagtggttttggaaggctgtgctgatgatggatgcagaaaagaggatt
cgattacttcagtttgtcacaggcacctctcgtgtgcccatgaatggatttgctgagctgtatggctccaatggaccacagttgtttacagttgaaaaat
ggggcactccag
3' EST: DT390312 [+]ggaataattctttattgcttgttagttgctcttgtgcacattttaaactacaaagtatgaaaacaatcatttgacacttgaatctatacatatgcaagtt
tcccataatagatttatacaaaatattttgctgtgtagttaaacaacttcattctctataaattatacgttatttacaagtaatactttcaggaataaag
agcaagcccactggaacaatatgaatatcggattacataaaatcccattaagacttcatacgcatgtagaactgaagtaaggtgcttaagggcagcaata
ccagttactcagttactcagccaagctcatggtattgctgaagaaaactcttaaaaaaagaaaataggtttgtaggtcacagtgtcttcgtgaatcagcc
agatacattagctccatattaacacaaatacctgtatatttagctgaacaactaaatgagggtccaataaatgtcactgctgcttcaaaggttcaattgc
atatttcactttactgcatagctatataaaaagtcaacttgtgcttgcctacatgaaccactgtgaaggtgggaggcctgtgtaataccagggagcaggc
acctaaacattaaagacaagtaaatgactaaatccaagaaaaatgacaaggaatatgaattggaaaaaagtagattagcactaaaataactgttaaatag
tttctcacttaggaaaaataatttatatgtagaaaaagacaatgagccatattaagtgacaacaaagactacaagtattttcacattattacattaattt
tggtaatgctgaattgtagcgacttatctagtagcaaggctaaaaataaaagctaggatatttatataaaaaacatcaactagaatacctgtaaacgtat
a
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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