XB-CLONE-3186145
Clone Name: IMAGE:7851329
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Gene:
ogfrl1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC128651 [+]
GCAAGCCGCACACAGCCACGCCAATGGGGAACATCTTCAGCGTGGCCAACTTTAAGGAGCCCACTACGGTAGAAGACTGTGACTCCACATGGAATACTGA
CTCTGGGGGCGAAGAGGAGCAGCAGGAAGCCGAGGAGGAAGAAGAGAGCGAGGGGACAGAGCAGAGAGAAGACGATAATGAAGAAGAAGTCATCAAGCAG
GAAGAAACCAATGAAGGCGGCGAAGAGGAAACTGGAACTGAGACTCTAATTGCAGAAGTGAAGGCAGTAATCCATAACCCAGAGCAGGGTGACAGCAGCA
CAGTGGAACAGAACATCAAACCAAAGAGAAGCTTTTATGCAGCGAGGGATTTATACAAGTATAGACACCAATATCCACAGAATATTAAGGATCTTCGTTC
TCCAAATGATCTGTGCAATCTGAGATTTTACATGAATAAGATCCCTTTCAAACCAGATGGTGTTAATATTGAAGAGATTTTGAATAAGTGGAAAGGTGAC
TATGAAAAGCTGGAACATAATCACACCTACATACAGTGGCTTTTTCCTTTAAGAGAGCAAGGTTTGAACTTCTATGCTAAAGAACTGACTTCATATGAAA
TTGAGGAATTCAAAAAAACTAAGGAAGCAGTTAAAAGATTCATCGTGGCGTACAAAATGATGCTGGATTTTTTTGGGATTGAACTTAGTGATAAAAATGG
AAATGTTTCACGGGCACCAAACTGTCAAGAGAGGTTTCAGCACTTAAACGAATCACAGCACAATTATTTAAGAATTACCCGGATTCTGAAAAGTCTCGGA
GAGCTTGGCTATGAAAACTTTAAGCCACCACTAGTAAAGCTCTTTCTTCAGGAGTCTATTGTTGCCAACACTATTCCAAATATGAAGCAGAGTGCACTGG
AGTATTTTGTATACACCATTAAAGACAGAAGGCAAAGGAGAAAACTTTTACGTTTTGCCTGCTATCATTATGAACCGCCTGAACACTTTATATGGGGACC
ACCAGATAAACAGAAGGCAGATGAAAACAAAGCTACTAAAAAAACAACCACGCCAAGCTCCCAGAAAAAACACTCTCATGTAGAAAAAAAATCAAGGCCT
GCCAAAAGCATCAAAGCCCCAGAGAGCCCAGCAGTTCAGCATGATGAAGAAAAGGGAACAGAAACCATGCAAACTGCTGAAATTCACAGACAGGCAACAG
CTGAAGTTATTTCTGTCAATGAAACAGAAGTTTGTGATGATGGAACAGTAACATCAGAGAATAATAGCTCTAAAACAGGGCAAACGGAAGCTTGTGATGA
TGGAACAGTCACAGCTGAGGACAGTAGCTCTAAAGCAGAGGAAACGGACTCTGGCAATAGTGAAACCAGGTCTCTGGACACAGAACATGACTTAAAAAGA
CCTGAAGCTGACCGTGAAACATGCTGTAAAGAAAACATTGTAGTAGTAGAGTATACTGAGAAAGAAAGCAAAGACTGTTTGTGCAGCTTATCGCCTGGTA
CATCAAACAGTAATGTGACCGAGCTTAAAGTAGAAGGTTCAGAAACTGGACCTTTTACCTAAAGATCTAGCCAAATAAAAGCTCCAAATGTTACAATATT
ATTTTTCACATTTTGCCCCTTTATGAGTAAAAAACGAAAAGTGTAACATTCACTGTCACTATCTAGGATGTAATGTAATCTTTATTTAAGGCCTTAAGTG
AATCTCTAGGGATACAGTCCATTTTTCTCCTGCATTACTATAAGTGCCCCGTGCCAAGTCTGTAGACTTATAGTCAGTCTAACATTTGGGAATGAAGAAA
TCCTGGCTCGCTGTGTTTAATGGTGTGTAGCTAGTGGCTGCTTGGTTCTTATAAAGGCACCACACCAAAAGACAAATTAGCCAATTTGACACACTGAAAA
CTAAAATAAGCTGGTGATTTTCATCCTGAATGTAACATGACCATTCCTGTTTCTGCTCGGTCTGTGCTCAAAGGATACATCATGTAAATGTAAATTATAT
TGAGCACGCAAGAAGCCCATAGTGCAGAATTTCTTGCAAGAAAATATTAGAGTCTAAAAAAATTTTTTTTCTGAATTCATATAGGTTTTTGTACTTCTGT
GATATACGCAACAAGAATTGTAATTATTTTTGTATATCTATTAGATAAAAACGTCTCTCCAAGTTAGCGTAATCTCTTTGTAATTAGGTAAAAATGACTA
GGAAGTGTTCCTTTTTATGCTTTCTGGATGTTTAGCTATGCTAGAGATTAAGTAAACAACCACAGAATGCATTCATATGTTTTATGTTTATTTATTTTGC
CACAATATAAATGCAGAATGGATGAGCTAAGCTTTCATTAAGGGCAATGGCACACAGAGCTGATTCTGGCACTGTGTGCCACATTTTAAGTAGCTCAAAA
ATAAGGTGTCAATATGCCCCATCCCCCCCAGTAGTTACTGACATTGCCATTGCTTGCAACAAGATATTTACCTGGAAATGCATCTATGTGCCAGAGATGT
AGCAGATTTTTTTTTCTATATAATTATATTAATCTATGGTTTTTCAAAAGAGTTTTCTAATGTACAATATTCAAACATTCTGGTCATTCTAAACTGGCAA
CTGAACTAATTTAATATGTATATTGTGCCACCCTTCATCTAAATGATGATTAAGCTGATTGATAAAACAGGTCTACCAGTCGGGACCCAAGTTTTCTTGC
AAAACTAGGGTCTCAGTACTTTGTACAATCTTTTCTGCAAACTACTCATCCATGTAATTGCAAGCAACATTACAAAGGCTTCCCTTTGCACTGTGTGATA
TATTAAAAAAATCAAAAATATATCTGATCCTGTTGTTTCCTAGTTATCTAGCTTTGGGGAGGTGTCATTCTGGAATTTTGTACAAATAAGTTTAAGAGTA
TCAATTTAGCACTTCAGAAACAAACAGTGTGGAGTGCAAAGGCTTTGCATTGCATCAGACAACTACATGTTTGATTTAACAACTTTTAGAGCCATTACAA
ATGACTGCATCTTTCCCCAATATGTCCACCTTGAGGTGATGGGATACAGGCCCTCAGGATGAGGACCACATCATTGAGCTGGCATGCTCCTTGTCCCCAT
GAGATTTCTAAACTTGCGTGATCAACATATGGCCAATATTCTGCCAGATATTGGTCCGTAAAGCTGGCCATACACGCACCGATGATATCATCAATAATTT
GCCCCACCATATTCTGTGTATTGCCATCTTGAAAGACAATTAAATCCTAATTTACTGGGGGTGGCAAAATGTGCACATGCAATTTCAAATTGACTTATTT
TAAACTACTGGCAATGTGCAAGTCTAGTTCTTTTCATGTGACAACTGGGAGAAACACCAAAGATGATGCACCAGCTTCGGGAAGGTGAGGGGGTTTCCTA
AAAGCATGATATTGTTGAAGCCAGTTTTTAAAAAGCAGCAGACAACTTCCTAGTGGGATGAAATAAAAAATATGGAACCTGAGGTTTCTATTCAAACAAT
CATCTTATAATACTGAAAGTCAAACACCATGCTTTCAAACATCATCAAATTGTCAATTGTTATCTTAAAGAAGAAGGAAAAGAACTAAGTAAGCTTTATC
AAGAAATAAATACAGCCATAGGCATTCACAGAAAAGCTGCACTGAGTCCTCTATCAAAAGAAACACAGGATTTCTTCTTTTGTGTCTCAGAAAAATCCTT
CAGGACACGGCACAAGTCTGCTCGGTTTGCTCCTCTTACCCCCTCCCTTCTCCCCCTCCTTATCTCTCTCCTCTTCCCAGCTGTGCTGTGTAATCTGAGC
TAACCAGCAGTTAGAGCTGCAGCACAGAAGCTTCTGAGACCGAACTAAAATAGCAGCTGCTATCTTAAATATACAGAGCTTCTAGGGCTATTTACTCAGG
TATAGTAAAGCTTTCTGCAGAATAAATATAGTGTTCTAGGTGGCACTATTGTGGCAGTAAAATGCCAAAATGCCTTTCCTTCCTTTAAGTGTCAGATAAA
AAGGTATCCTACAAATGCAGCTGAGACATGCAGTGAATTCAGAAAATTGTAAAAAGCTGTAAATTAATATACTGTAGATCAGGGGTGGGCAAACTTTTTG
GCTCAGGGGCCACATTGACTTACCGATAGGTCGGGCCAGCGTTACGCCGTTTCCGCTTGTTAGTCCCCAGACTTTGTTAGTCCCCAAAGTCTGACGTGAT
GAGACTTGCACAGTGGGCGGGCTGGATTAAACAGACCAAAGGGCCAGACGTGGCCCGCGGGCGGTAGTTTGCCCACCCTTGCTGTAGATGCTGTGGTTGC
TAGGGAGCAGGTTTGGAATGAAGCAACTGTAAATGTATAGAAATATTTATCAAGTTGTCACGCTTGTTTGCCCCAGATTTCGGATTGGGTGCTTCTCTCT
GGCCAACTCTTTCTGTTCAAATGGGATTTCTTTGTGCTGTTTCAGTACAAGGTGAATACTAGATAAGTTTGCATTGCTTTGTGTGTAAGGATAAAGTTGT
TTTCAATGCATTGTTCTTGTACATTCTGTAGAACTGACAAGAATACGTTCCAGAAGGCTTTGCATATTTCTTCCAACACCATCTGTTTGTGGCATTAGCT
GTAATTTGTTTTTGGAGTTGTGGAACAAACTTTCCATTTTATAATTTCTGTAAATGAGTTTTTACTCTAACTGAAAAGTTATAGAATCTGTTGCTTCTTC
CATTTTTGCAATTGGGCTAAACTTGTATATTTACAATTACCTTTAGCTACATTTTTTACAGATGCAGTCATTGTAGAACAATATAAATAAAGTCACAAGA
ATTGATTTCAATTGTCCTGTGGTGGAAGGAAATATATAGCAGCTTGCCATCCACAGTATTCATTAATCACACTAAAACAGTACATGGTTTATGGTCTCTG
TATTCCAGTCCCAACTGTTTTTCAATAAAGTATTTTTCCAAAAAAAAAAAAAAAAAA
5' EST: DT390406 [+]caagccgcacacagccacgccaatggggaacatcttcagcgtggccaactttaaggagcccactacggtagaagactgtgactccacatggaatactgac
tctgggggcgaagaggagcagcaggaagccgaggaggaagaagagagcgaggggacagagcagagagaagacgataatgaagaagaagtcatcaagcagg
aagaaaccaatgaaggcggcgaagaggaaactggaactgagactctaattgcagaagtgaaggcagtaatccataacccagagcagggtgacagcagcac
agtggaacagaacatcaaaccaaagagaagcttttatgcagcgagggatttatacaagtatagacaccaatatccacagaatattaaggatcttcgttct
ccaaatgatctgtgcaatctgagattttacatgaataagatccctttcaaaccagatggtgttaatattgaagagattttgaataagtggaaaggtgact
atgaaaagctggaacataatcacacctacatacagtggctttttcctttaagagagcaaggtttgaacttctatgctaaagaactgacttcatatgaaat
tgaggaattcaaaaaaactaaggaagcagttaaaagattcatcgtggcgtacaaaatgatgctggatttttttgggattgaacttagtgataaaaatgga
aatgtttcacgggcaccaaactgtcaagagaggtttcagcacttaaacgaatcacagcacaattatttaagaattacccggattctgaaaagtctcggag
agcttggctatgaaaactttaagccaccactagtaaagc
3' EST: DT390405 [+]ggaaaaatactttattgaaaaacagttgggactggaatacagagaccataaaccatgtactgttttagtgtgattaatgaatactgtggatggcaagctg
ctatatatttccttccaccacaggacaattgaaatcaattcttgtgactttatttatattgttctacaatgactgcatctgtaaaaaatgtagctaaagg
taattgtaaatatacaagtttagcccaattgcaaaaatggaagaagcaacagattctataacttttcagttagagtaaaaactcatttacagaaattata
aaatggaaagtttgttccacaactccaaaaacaaattacagctaatgccacaaacagatggtgttggaagaaatatgcaaagccttctggaacgtattct
tgtcagttctacagaatgtacaagaacaatgcattgaaaacaactttatccttacacacaaagcaatgcaaacttatctagtattcaccttgtactgaaa
cagcacaaagaaatcccatttgaacagaaagagttggccagagagaagcacccaatccgaaatctggggcaaacaagcgtgacaacttgataaatatttc
tatacatttacagttgcttcattccaaacctgctccctagcaaccacagcatctacagcaagggtgggcaaactaccgcccgcgggccacgtctggccct
ttggtctgtttaatccagcccgcccactgtgcaagtctcatcacgtcagactttggggactaacaaagtctggggactaacaagcggaaaacgcgtaacg
ctggcccgacctatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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