XB-CLONE-3186136
Clone Name: IMAGE:7851521
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390412 [+]
ccatcgattcgcgcccagatccccaaaaaccacgtcagcctggccactaaggagaaccaactctttgtagctggcggcctttattataatgaggaaaaca
aggacgacccactgagctcttacttcctgcagttcgatcacctggattctgattggctgggaatgccccctgtcccctcagcacggtgcctgtttggtct
cggggagtcggacaattccatatacctgattggtggaaaggaactaaaagaaggagaacagacgctggacactgttctatgctacgaccgtccgtcattt
aaatggggagagtcggacccgctaccctatcaagtgtacggtcacaccgtggtgtcccatgataatctggtctacgttcttggaggcaaaggaaatgaaa
agaaatgtttaaaaagagtttgtgtctacaaccccaagaagtttgaatggaaggacctagcaccaatgaaaaccgctcggtcgctattcggaagcactgt
ccacaagggaaagatcttaatcgccgccggagttacagacacgggtttaaccaacaccatcgaagcgtatgatgtcaagaccaataagtgggaggagttc
acagagttcccccaggaacgtagctccctcagcctcgtcagcatgaacgggaccctttatgctattgggggatttgccaccacggagaacgaatcggggg
agtttgttccgacagaactgaacgacatatggaggttcaacgaagaggagaagaaatgggaaggaattcttagagagattcgatatgcgtctggagcgac
cttcgttgcagcgcggctcaatatcctgcgactaacgaagatgtaacgacgcgccaggcaatggaagcttaggccaggatatagagaacatgaacgaact
atggggcttactgatggtgggacatgttgctgggtcaaaatattcctaggccc
3' EST: DT390411 [+]agcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaatc
cttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgacctt
tattgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaattta
ggtacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttgaa
aatcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaat
ggttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaaga
ttcatccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatgat
ataatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtagaaatgacagg
gagttgatagctacaaactgtgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: