XB-CLONE-3186023
Clone Name: IMAGE:7851246
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Gene:
cyc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390486 [+]
cggaggtcgcttggtggggcagtgctgcaggggggacgggcggcctggccggtggctcctcaggccaacatgtccttctccactctgtcccggggcagga
aggtggctctctccacattaggtattttggtcgccggaggctccggtttggcgtttgcactccaccaatcagtgaaagccagtgaattagagctccaccc
accctcctacccatggagccacaacggactcctctcctcgttggatcacgccagcatccggcgcggctaccaggtgtacaaacaggtgtgcgccgcctgt
cacagtatggaatacttggcctttaggaacctcatcggcgtctcccacacggaagcagaggccaaagcgctggcggaagagtttgagattctggacggcc
cggatgagaatggggagatgttcacccggcccgggaagctgtcggattatttccccaagccgtaccccaatgacgaggcggccagagcgtccaacaatgg
ggcgctgccccccgacctcagctacattgtcaatgccaggcacggcggggaggattacgttttctctctcctgactggttactgcgacccccccgctggc
gtcagtttaagagaaggactgtattacaacccgtacttccccggccaggccgtcgggatggcgccccctatttataacgaggtcctggaatttgatgatg
ggaccccggccaccatgtcgcaggttgctaaggacgtctgcacattcctgcgttgggcttcagaaccggaacacgaccagcgcaagcgaatgggccttaa
agtgctgatgatctcttccatcctgatcccactcatctactacatgaagagacaccgatggagcgtgct
3' EST: DT390485 [+]ttttttttggtttaaagatatatttttgtttaatttaacgagcccataatgggatgtaatggacacaatcgtttcttcgcacgagtccctttttcctcct
cggtttggttctttccggacaaaaacagtgaaaacgcagagaagcaggaacaaaaaattcccccgagacaaaaaataaacataaaaaaaacaaaaagata
cggacgcagcgcctgtgagttacggagccgtgtccggatctacttcctgctcggaggagcaaagggaccgttgcctggcaaatcatccatggcatctgct
gaggccaacgcgcagggggaggggcttcccggtgctgagctgtcggctgccgttacttggggggccggtaggcaatcttcctggacttcagcacgctcca
tcggtgtctcttcatgtagtagatgagtgggatcaggatggaagagatcatcagcactttaaggcccattcgcttgcgctggtcgtgttccggttctgaa
gcccaacgcaggaatgtgcagacgtccttagcaacctgcgacatggtggccggggtcccatcatcaaattccaggacctcgttataaatagggggcgcca
tcccgacggcctggccggggaagtacgggttgtaatacagtccttctcttaaactgacgccagcgggggggtcgcagtaaccagtcaggagagagaaaac
gtaatcctccccgccgtgcctggcattgacaatgtagctgaggtcggggggcagcgccccattgttggacgctctggccgcctcgtcattggggtacggc
ttggggaaataatccgacagcttcccgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: