XB-CLONE-3185947
Clone Name: IMAGE:7851492
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Gene:
mrps9
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390536 [+]
ctgtagtgtgccgtggtctagggtttgcagctgtattacgggcagccggtacctgtagtaggaaatcgacattgactgctggagcctgtggcgaggcttc
tcgactaatacataacagtgctgcagtacacaggaaaaatgttgcagccccaggatgcgaaaagtacacagaggcctttattaaaaaacagattgaagag
tacaacattggaaagagacatttagccaacatgatgggagaagatccagagacatttacccaagaggatattgatagagccattgcatatcttttccctt
ctggcttatttgataagaaggcacgaccaattatgaagcatccagaggacatttttccaaaacagagagcaattcagtggggagaagatggccgtccatt
ccatttcctgttctatactggcaaaccatcatattactcattaatgcatgaactctatggcaagcttctatcaatacaaaaatatgaagaacaaatgaga
agcaaaggtttatacacagaaaatacaaaacagatggacctgattggcagtcgctggttgattaaagaagaattggaagaaatgttagttgaaaaattgt
cagaccaagattacctgcattttctacagatgctggaacgcttattgattctgccatactgcagcatcgaggaagaatatgtgcttaaattccgtaagaa
ccttgaggttcagtccaaaaagcaggtggttaaccctttgcagtatgatgaacatggaagggcattcagtactggagaaggtaagaggaaaactgcactg
gcaacagtggtgcttcatgaaagt
3' EST: DT390535 [+]atttaattaataattttattataaaaaaacagnctttggaaacaacagaggaagcagcttgctcgggaaagcaggcacatttccactgcaatacagttga
ggcttaacgtttcttccatgtaaacttcctgcgagctccttcctggccaggttttttcctttccttcacacgtggatctgtagtaagcagcccagcttgc
ctcatggcttcaatttcattttcagatacaaagctcagcaaggccctagaggtggccaggcggatggcaccagcctgtgatgacttccctcctccagcca
cagtgcattctaagtcatgctttccaagccggtcaaggaattggaatgggaacattagctgctctctgtcctgcagtacagtgaaatattctatataatt
tactccattcacgaccattgttccatttccactttcatgaagcaccactgttgccagtgcagttttcctcttaccttctccagtactgaatgcccttcca
tgttcatcatactgcaaagggttaaccacctgctttttggactgaacctcaaggttcttacggaatttaagcacatattcttcctcgatgctgcagtatg
gcagaatcaataagcgttccagcatctgtagaaaatgcaggtaatcttggtctgacaatttttcaactaacatttcttccaattcttctttaatcaacca
gcgactgccaatcaggtccatctgttttgtattttctgtgtataaacctttgcttctcatttgttcttcatatttttgtattgatagaagcttgccatag
agttcatgcattaatgagtaatatgatggtttgccagtatagaacaggaaatggaatggacggccatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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