XB-CLONE-3185603
Clone Name: IMAGE:7851474
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Gene:
c6h6orf62
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390763 [+]
gagtgtgcgccggttatcctgcaagctccctgtcggctttagctgtgcttaggctgttgtgtttaagggttgaacgtttgcgtggaaccgaggcctttcg
cctgctcttgattatcagcctcatcgttctcgtcatctccttaccccgtcacagcgcggcaccagtccggactatgggtgggtcggggtgtattagggtg
cgcagcccactgagcagtgcaggtctgacgcagtggggtccgttggggtccctggtcttttcttgacatggatccccctggagcactcaccttcaccacc
gccagcctccccctacagagacagctgccccggttccctaatacccacacccagtctctcatagacacccggacagacatgtcaggcagccagcgcgact
aggacagcagaacagccacccccgaacagagtatctcctcctcacttaggatttctctttagcgctagagttcggaatgatcacgtgtggcgacatgatc
gctccaactcgccgctaagctttataatgggagggcggacatgtcagtgactagtgataggggaactgcgtagtggtacaagcctgtttcggcccagtat
acttctgtagcacttgctcactggcatcatggtgtcccttcccaggggctgtcagctgcactccaacatggtccccatctctggccaaatccccggaatc
gtggggaaactcaacttgtgctgtgaacaattcctctccccctaccacacagataaataatatagattctcacggtatacaagctgtagacggggagcta
ggggctgttgacctaaagcgagctgacatcattggag
3' EST: DT390762 [+]agatttcaactcatttattttaatttccaatgtaatccaatttcaaacaaaagctagatacaattttaaaccaggtaagcactagtattagccaaaggga
ataggcctatacaagttgattcataaaaggtactgaaaccttttagaaaagccttctcttaaacaatacagtagtcaaccttatcattgcacaaaataaa
atgattctttgaaccaaatttcacacttcaaacttcttttacaatttgtaacggtgttctgacattactttattgaaaccaccaagggtttttctttttt
ctatagaaatattacttcattttatgaaaaagtaatatttgcacaaatcaccagcaggtttctatttcagttcctatcttcagaaacatggtttctgagg
tgtcaacgaattttaagtcgttttcatatagttactgcagactcctagacttatgtcagtacccacaggtaggggaactctgttatgcagcaactgggag
aatataccaaagtgtaaaagtgccattactcgttcactgccctgcaagctgaactacgatatgaaagcaaatgctgaataaagtttctttttcaatacat
ctgagccagtcttcattgtaagccaacatagggtatgctttaagagattcagatagtgctttcataatacagtgaagcagcaaagctatttatacaactt
acattcattttttttcaccctcaatactgtcatctaaatgatgttaggggaatgaccagtgcaaatgggaagaccactaaacaaaataccttgtcatttc
agatacatttgaatca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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