XB-CLONE-3185000
Clone Name: IMAGE:7851863
|
Gene:
psmc5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT391162 [+]
ccgtggaggtattggcctgaggcagtattacttgtccaagattgaagatctacagcttgttgttaatgacaagagtcagaatctcaggcgcctgcaagca
cagaggaatgaactgaacgctaaagtgaggctactgagggaggagctgcagctgcttcaagagcagggatcctacgtaggagaagtggtcagagctatgg
acaagaagaaagtattggttaaggttcaccccgaggggaaatttgttgtggatattgataaaaatattgatatcaatgatgtcacaccaaactgtcgagt
cgctctgcgcaatgacagttacacactgcataagatcctgcccaataaggttgatcccttagtgtccttgatgatggtggagaaagtaccagactccacc
tatgagatgattggtggcttggataaacagatcaaggaaatcaaggaggtcattgaactgcctgtgaaacatccggagctctttgaagccttgggtatag
cgcagcccaagggtgtgttgctgtacggacctcctggtactggaaagactcttttggctcgtgctgttgcccatcacacagactgcacatttatacgagt
ctctggatctgaactggtgcagaagttcatcggagaaggtgctcgtatggtgcgtgagttgttcgttatggcccgagaacacgctccctccattatcttt
atggatgaaattgattcgattgggtcctctcggctcgaggggggctcaggaggagacagtgaggttcagcgcactatgcttgagctgcttaaccaactgg
atggat
3' EST: DT391161 [+]aagtttgtttttttgtatttatttcacatgagataacatacacatatctgttacaaaaccaaaatcccgccgtactaaatgcccaatggaatagtctaat
ttctagagaaagcgtggtctcctagaataataggcttcagggtgcttccatattgtggctcccagcattccctgttagcaaaaaaaaaaaactggaaagc
caggtgttaaagatctttagattacaggagtttgtgttgagttttctgataatttactgtgagccttgttgggaacgctgggtttaataatcaaaacagg
ctgacattacagggcttcagctgggagcagagtgtaatgggaaccgaaaaaaaaatcacttccacagcttcttaatggacatgttcttctcgctgtcttt
ctgcatcacctttgctacagccatttcaaagtcttcttgtgtgacgtgcaccctcctctcgcgaagagcatacatccccgcctctgtgcacacccccttc
acttcagcccctgatgccccaggcatcagttctgctattttacgcaggttgatcccacgagtcaagttcatttttcttgagtgaattttcaggatgtcca
gacgggcctcttcattaggaggtgggaactctatcttcctgtcaatgcgacctgggcgcagcagagcagagtccaatatatcgattctgttagttgccat
gattacctttatgttctttgttgcctcaaatccatccagttggttaagcagctcaagcatagggcgctgaacctcactgtctcctcctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: