XB-CLONE-3184703
Clone Name: IMAGE:7851604
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Gene:
rasd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391356 [+]
tcatgaacttaagggcatcataaagcagatctatcccccagttactgagacaatatctataatgacatggcttcttttgtgagaagtatgaaatcgcttt
tattcggctacagtacattgatttgcagctttcatagcaaatcaatggcacaaaagaattgcgtcaaacggcagagagtgcagggttgcatctgcaagtt
catctacaatgaggcagaatgaaacaggggatgtagggaaggtaataatgtttcattctggatgtaagagaagtgacctgggccaaggtatctgtaagtc
attctgtagatggcaaaattagtgatacaggtaatcagcaaaggtggtatagaatcatgtagtaaagcattcagttaacaaaattctgccagtcagtctg
tattgctggaaaatgcacaatattaccagcctccaataacaaaaaataaataaagaatttgttaaagcaaactgaaactggtgtttcagttgtacacatt
tgctctttctataaaatatatggttatatctctctgaatagattggtgcccattttgctaagcaatatttttaccatatgccccagcctatccttagctt
gtactgtagggttttacaaagtctgcctgttttattactgtaatgccccctatattacattgcttgggttcacattgctctgcatatctcactatattaa
tgctatgctctggatgtctatgtatttactttgcctgacattacattgctctgcatgtgttcctatagcactgaaaatcctctttatgtttc
3' EST: DT391355 [+]ttttatattatttatatagcgtaaagtttattttgcttaactaacaatattaaaaataatttggagctcttagggtgaaaggtcccatttaagatatatg
tcatagaaaacagacacgacaactaatacagggcagttcagcttataacacaattataaagcatcagaccagatacaaacaatataacaaatacaaatga
agaataaactgtagaaagaaagacccctgtttgcaagagcttgcaatctaaaggctgcttgattgggacattacagccttttttcccccagcaacaaaca
agtgggccctttgctatgaggtggtcagtgggcattttttttagggcttaatgagctgtgcccatggggctgtggggatctgtgcgatcagctttacatt
aaacgtggtttaattctatttaataataatgcacatgttacaaaaagagttctctctttacccagcaatcagtgttttatagaaaaagcaaacactttct
agttgtaacaatatgaggatcaataaaaaaagtgcaagctcttcggggaaagtattgggagacagataggtgtttgacataaaatagcctaaagaagaag
caagtactttgtacagcagcccatctgtgtattccggctctcaggaaagccctgctgacatctgattggatagggcccttgtagggaggtgggccatgga
ccttgggaaaaagcctgtcctttcagttcaggagacagaagagacacaagggtcctgctggaagctgcacactgtttcgtgcctgggactttgtggattg
ggtgccaagagaaggagacaaagagaaaaactaggcgctgttctgttactgagcagcacagagaggatttgtga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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