XB-CLONE-3184429
Clone Name: IMAGE:7852059
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391537 [+]
cacagatacaccctgcctcctgatcaccccgactttatccgagctcgtatcaacgcctcccagatcagcgataaagcctataaaacctcgtgggagcagt
cgcgggccgctgggtacgacctgagactcgacgccattcccttccaaaccgccaaggtgtccagggagatcgccagcgatttcaagtacaaagaagcctt
catcagggagaaggggcggcagattgggttccgcagcatcagcgacgaccccaacgcccaacacgtcctgagggtcagcaaactgcagagcgacagggag
tacaagaagctgtcggaggagagtcgcccccgagtcagaaccaacctggaccaaccgggtttcatccaagccaaaaagagccaggaacaagccagcgaca
tccactaccggcagcacttgcaccgctacacgtgtgacccccagcagctagccatgacacacgccaagcaagcccacagcctgcagagcgatattaagta
caaatcagatctgaactggatgaaaggggttggttggaccccccctggctactacaaagtagaactggctcgccgggcggcccaactgtcatacggagat
ggcacagagtcagaccgggcgtcagtgtctccttattcagcagacgagacggggtaccagtccccacacagcgatgttaatcccgatgcctccgagatcc
ttcaggtgaagaggaagaaaattctggcaaaaaagaaatgaacatttggtgccggtccgatcgcttgggaattccccggggcagatagaaaggaacgggg
cagctcctgctatgtttgtgtgtaactcagggtatttatcatgactgcctgggttattctgcccccgtgcacaaggaacgtcctaaactgccccccctgc
tttgccctactttg
3' EST: DT391536 [+]gaatggagtcgcgtttattccgtacaaacgattctacaaagaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccgatatacgtg
acaaacaaacacaggggggtgtcggctctgcaaggtcagactcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctattaaactac
actgggggggcaattaaatgtaaagaaaccaaggggggcagtttatatgtggcattaacagcataactgactagttccccactagtaatactattatata
gaacccccacacattctaccacattctgcctcctgcggtggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacgggggcaga
ataacccaggcagtcatgataaataccctgagttacacacaaacatagcaggagctgccccgttcctttctatctgccccggggaattcccaagcgatcg
gaccggcaccaaatgttcatttcttttttgccagaattttcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgtggggactg
gtaccccgtctcgtctgctgaataaggagacactgacgcccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagccagttctact
ttgtagtagccagggggggtccaaccaacccctttcatccagttcagatctgatttgtacttaatatcgctctgcaggctgtgggcttgcttggcgtgtg
tcatggctagctgctgggggtcacacgtgtagcggtgcaagtgctgccggtagtggatgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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