XB-CLONE-3184316
Clone Name: IMAGE:7852069
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Gene:
syap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391612 [+]
tgacggtcggattagaagggaaggggagaataataaacatttttagatagtgttgtgggaaagctgtaaccctgcaatgtttcgcggacttgggagctgg
ctcgggctggacccggagcaggaaacacaaaaaacagcagagaaggaaacaggaaggaatgaagaagagactagaagcgcggaaccaggagacggggaaa
agttgaaaagcgctggggaagatgggcagtctcagcaagaggcaaatgagtcagacgaggccctcataaaccaggccaaaggctttggcagttacctttt
aaactttgcttctgttgctacaaaaaagatatctgaatctgtagccgaaacggctcaaaatatcaagaaatcggttgaggaaggaaaaatcgacggttta
attgataagacgatcattggtgattttcaaaaggagcaggaaaaatttgtacaagagatgcattttaaaaagtcagaagctgcggttgctccttgggttg
gttataatgaagaggaaaccattcaacaacaaatactggccctatctgcagacagacgcaatttcttaagagatccccccgctggagtacattttaactt
tgagtttgagcaaatgtaccctgtcgcccttgtaatgttacaggaagatgagctacttaaccgaatgagatttgatcttgtcccaaaactcgtaaaggaa
gatgtgttctggaggaactacttttaccgtgtttctttgattaagcagtctgcacagttaacagccctggcagcacagcagcaagcaacaggaaaagctg
ataagattaatagcatgncagatggcggtgactt
3' EST: DT391611 [+]accataaattatttatttgtctgtataaacagatcataaaccaaactggaatatacatttaaacttgtgcagaggaaaatcttaattgattaaaatcagg
tataatgttctttatggcagggtaaaacaaaaatatgggccaaaaaaaaaaaccccccaagaaacaaacaaatccagacaggataccatatacctttcaa
atcacatagaaccaggcctcactcattttgtacgtattggaatggaaaacaaatgcttcgttttgaatggtgaaaaagaaatcgtgttccaataatttgg
caccacacacctctgtggcaaatacttggtcttttagcttttacttgtactagagagacatatatatgatgaaaccaccggcgcctctctggcaaagact
ttcttcccaggctggaaataaatacgttaaattacatgtcagctctacttcagtccatatcccagataaatgaataagaccgctgtgttgttacattgtg
aagtgctttttaaaatagattattacagtcacaatccagaagagacagggcttcacagggcttcccagaacttcagtttaatagcacttaatcttccatt
gctagttctaaacagtagcgccttagcagtacatagcgaaaacagccatgatcttattctgtggtttgcagagcctgaaggaatgactagagtaggaaga
gaatagtatagacacagtccaactgatcagcattacgttagtcctcttgtaggagttcctcaatctctttgtcccaattttcatccttcattttgtcaga
gtctgtcaccacctcatattcttgaaccacatcctgtaactcctttatccagtcctcggtttcttcttctggtttatcagcctcatcttttttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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