XB-CLONE-3184175
Clone Name: IMAGE:7852177
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391705 [+]
ctacagcacccngctctcagatagtagcaccatgcctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcatt
gtggccactggcaagggcatcctggcagccgatgagtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgcc
gcttataccgccagctgctcttcaccgccgacgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacga
tggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaacc
acaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaac
acactccctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccga
ggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctac
ctggaagggaccctgctgaaaccaaacatggttacccccgggcacgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccc
tcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggtcagagtgagga
3' EST: DT391704 [+]cgagaggctgagtgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgc
acgtagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtcc
gcagtctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccct
cactaggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccg
cctctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcc
cccccaggctttgagggcggaggcctgaagggcacgtgccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtg
cacggtggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagca
acctcctgaggggggtacttcttggtgcaggcgtgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagag
ccttgtacactgcagccaggaccttctctgtcacatactggcact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: