XB-CLONE-3184146
Clone Name: IMAGE:7852275
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Gene:
idh2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391724 [+]
cggaaatctgacaagcttcctttctctcacccaccggggttctacttttgtcttcccgcctccttctgccttgttatctttcaccagaagagttaaaaat
ggcaggctacctgcgagctgttagcactatctgcagagcatccgccgccgccttgggcaagaaccctggcgtgctcgccccggccctcagccagcagcaa
ctgcagcagcaacggaactatgctgacaagcgtatcaaggtggccaatccagttgtggagatggacggtgatgagatgacccgtatcatctgggaattta
taaaagagaagttgatcctgtcaaatgtagatgttgagctgaaatactttgatcttgggcttccgtatcgtgaccaaacaaatgaccaggtcaccataga
ctctgcactggccactctgaaatacaatgttgctgtgaaatgtgccactattacaccagatgaggcaagagttgaagaatttaagctgaaaaagatgtgg
aagagtcccaatggaacgatcaggaacattttgggtggaacagtgttccgtgatccaattatttgcaagaatattcctcgtttggtacctggatggacgc
aagctattactattggcagacatgcccacggagaccagtacaaagctacagattttgtggtcgatcaggctggaactttcaagttagtctttgctccggc
aaatggaactgccacaaaggaatgggaagtgttcaactttcctgctggaggtgtcggcatgggcatgtacaacacagatgagtccatctctgggtttgca
cacagctgctttcagtattccatacagaaaaagtggcccttgtatttgagcacaaaagacactatnttgaaggcttatgantgcaggtttaaggacatc
3' EST: DT391723 [+]ttattttctgtacacggcaccaggtaagaggttgctgacaaaataaaggtgtattaaaaatttaggtccagcactaaacacaggcaactctaaggaccag
tcccattcagttgcggtactttagggtgtttaaaaattcgaccagcatttaatgggttaaaaaatttgttccagagccaagctattttggcaattcctat
tttgaatggttaaaaatgaggtaaaaaagttaacaggctgttaagctacagtatgttaattcttcccaagagctttgtcgaggttgttcttaattgcatc
caaaaagtctgtcgtgttcacataatgttcattaagacgaacgttgctcatgcctccatgaatacagccagccaagtcttttgtcatgatgccactttct
acagtctccacacagaccttttcaagtgttaaagagaagttaattaggtcctggtttccatctagcttgcctctgtgttccaagcctcttgtccatgcaa
aaatactggcaatagggttagtgcttgttggccttcccttttgatgctccctgtaatggcgagtgactgttccatgagctgcttcagcttcaatagtctt
tccatctgggcaaaccaggaccgaggtcattaaaccaagtgaaccaaagccttgagcaagaatatctgactgaacatctccatcatagttcttgcaagcc
caaacgaagcctccggtagatttcagaacctgtgcaaccatgtcatcaatcaatctgtgttcataccaaatcttcagtttgtcaaattctggcttgtaat
gcttctcaaagatgtcctggaagatgtccttaaacctgccatcataagccttcaaaatagtgttctttgtgctcaaatac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: