XB-CLONE-3184048
Clone Name: IMAGE:7852141
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Gene:
myoz1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391789 [+]
cttgttgctgccctctgagagacaagacacaagggagccaaattaataaaaaggaaagaatccgcaagaggacgtttacaattataacatcaaagaagct
cccatctctcctcctttagctgatctccagttcctgcgaaaaagaattaagaatttgttgtaatgccactgtcaggaacaccagctccaaacaagaggaa
aaagtcaaccaaaatcatcatggagataaatccatcaagagaaggagagatagaaatctccaaactcaatttagggaaaaagatcagtgttcccagagat
atcatgctggaggaactatccttgctcagtaacaaaggatcgaagatgtttaagcttcgtcaaaagagggtagaaaaatttatctatgaaaacaaccctg
acgtattttccgacaattccttggaccacttccagaaatttataccaactgtcggtggccaattaggggctaaaggacagtcaggtgttggagtaggtgg
ctatggattgctatatggcagtggtcagatgctatccagtatttcagggggacggcatggatctggtggtcagcaacctcctgtacctccaccaaaacct
ggcagtaagagcgctcctggaggccagtctggaactggaggaagtggtggaaaggattctggtgttgttgatggtagtcaaggaaaagagggacagtcag
gtcaacaggatggaggaagtgggaagcagatcactgtctttaagacatacctctcaccctgggacaaagctatgggcattgaccctaagcaaccaacaca
ttttactgtcaatttactagagtatggcaaaaaggcagagctttgcaaatataagtcatttaacaggagtgctatgcctt
3' EST: DT391788 [+]aatgttgtaaatgtttattcagtttaacattgcacaaatgaaaaatatcaaccatacagcatgactgaggagccaacagtaaacttatttggagactgtc
cttttatcgctgccacagtgcacaaaacattaagcttttttccttccactatcctaccttgtctattccatttacctctgcccagttccaactctgacta
acttccagctgaaaaaataccaccataacaaaaaacatttcataaacatacataaaggagctttgctgcattttgaacaaggcacattagagtgtggcat
agttgggtctaatgtgatctgccccattttttaaaaaaggcatataaagatttggcttaggtggtttagatatgacctgtgccattggctgctatggcat
cacagcatactgcaatgcattgcttgtaccacagatatttcagggatcaaccgccaatgtgtttgcaatatgttatgatgcagaggccagtggaactttt
tacatatgtcagttttcatacaccaacagactgctaacatggttccttggatgtatgatgagtttagagttcttctgtttctccatccactggcacagca
atatcaatgctgtagtttgtgttatcaccagatccaagccatccaattggggtcctgttgaaagaaggtctgttggtgatatcttgattgatgacaacca
caggctcaggctctggagggggggcatcaaaatctggcatctggaaagtcataagcttcgatgctttctcatatcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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