XB-CLONE-3184012
Clone Name: IMAGE:7852001
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Gene:
vps36
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391813 [+]
ccgaggcacgacgtctggcagaggaactgactcagagaagatgggaacgttcatcatcatcatcaccatctatgcaaatcaccaagggcccacagactgg
gagaatcaaggcagtgggaattgttggcatagagaggaaactggaagaaaaaagaaaggagactgacaaaaacatttctgaggcctttgaagacctcagc
aagttgatggaaaaggcaaaggaaatggtggaactgtccaaatcaattgctaccaagattaaagataaacaaggggacattacagaggacgagacaattc
ggtttaagtcgtatttgctgagtatgggaatagccaatcctgtcacacgggaaacacatggatctggcacgcactaccacatgcaactggcaaagcagct
ggcagcaatgttacaggctccattggaggagcgagggggcataatgtcccttacagaagtgtactgcttagttaatcgtgccagaggaatggagttgctg
tcacctgaggatttggtaaatgcctgcaaaatgctggaatctctaaaactcccaataaggcttcgtgtgtttgatagcggagttatggtgatcgaacacc
agtcccataatgaagaggaaatggtggcttctgctttagaaacggtttcagaaaaaggctcccttacctctgaagaatttgccaagattgttggaatgtc
tgttcttcttgccaaagaaaggctgcttcttgcagaacacatggggcacctttgtagagatgactcggtggaaggcttgcgcttttatccaaacctgttc
ttgacacagagttagttcatttaggttgctgtttcgagtgtggc
3' EST: DT391812 [+]tttttttaacattttcatatcaattttattatttaatatcacaaaaaatacattgcttatcaatccaatattatgaaatacatgttttaaccctcaacat
ccatagcaatattagaaagtaaggtcagtctagacatcattgatgcttttcattccccatctggttgcttgggttggattgactaacaaccagaaaactt
taacaaaccactgactgtagcaaccaaatagttttatttcgccaactggagaaaagtaaagtaatactagggcagggactaggggtaggcagagtctgca
tgtgcctagttggaggaggggcactagacaaatacctcttctgcctgactaccccaagcaggtccagactgggggtaggggagtgcctagggtgccttta
acttgtggccaagcgctgagtgatagtaacggttttgagggccacaatcttcttaagactagctggagtgtcaggatctgtaagtacagctgcatggtgg
tgctttcacacaacaagtaatctctgccacactcgaaacagcaacctaaatgaactaactctgtgtcaagaacaggtttggataaaagcgcaagccttcc
accgagtcatctctacaaaggtgccccatgtgttctgcaagaagcagcctttctttggcaagaagaacagacattccaacaatcttggcaaattcttcag
aggtaagggagcctttttctgaaaccgtttctaaagcagaagccaccatttcctcttcattatgggactggtgttcgatcaccataactccgctatcaaa
cacacgaagccttattgggagttttagagattccagcattttgcaggcatttaccaaatcctcaggtgacagcaactccattcctctggcacgattaact
aagcagtacacttctgtaagggacattatgccccctcgctcctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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