XB-CLONE-3183886
Clone Name: IMAGE:7851966
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391896 [+]
aagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaa
gcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcag
actggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatccca
ttattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaacta
tgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactcc
atcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccact
tcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagacctt
cctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcag
aagattgaagagatctttaaacaagcaggacaccccttcatgtgggatgaacatctggggtatgtccttacctgcccatcanacttgggcacaggcctg
3' EST: DT391895 [+]ttttttttagaggtgatattttggttttattgtctccaggcaatcattgggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatata
atctttatcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatccc
acccacatccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggtt
agggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaat
catgagtttcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgca
gtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacac
ctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttc
aatcttctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccac
accaggaaggtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgccagaggccagaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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