XB-CLONE-3183810
Clone Name: IMAGE:7852116
|
Gene:
ckm
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT391946 [+]
ccgaggggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgct
tctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctg
agagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaagagatctttaaacaagcaggac
accccttcatgtggaatgaacatctgggttatgtccttacctgcccatcaaacttgggcacaggcctgagaggaggtgtccatgccaagctccccaacct
cagcaaacaccccaaatttgatgagatactgggcaggcttcgtctgcagaagagaggcacaggtggtgttgacactgcagcagtaggaggtgtctttgat
atctccaatgctgataggctgggcttctctgaagtggaccaagtccagatggtagtggatggtgtgaaactcatgattgagatggagaagaagcttgaga
agggacagtccattgatgatatgatgccagcacagaagtagatgacaggggctagagacagagcctaaacaccctaacccttaccaccatcaaaggggac
aggagaccccattaagatcattagaggtcaaaaaatggccatctacaggggaatacataagtaatggatgtgggtgggataatctcagaaatgtagtagg
aagatccagcacaagaaaacaaaaacttcanaaatcacataaaagagaagataaatctaattctct
3' EST: DT391945 [+]agaggtgatattttggttttattgtctccaggcaatcattgggngggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttat
cagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacat
ccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtt
taggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtt
tcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaac
accacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctc
aggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttct
gaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaa
ggtcttgttttcattgtgccagataccacgggcatcaagccagtcacggcccatgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: