XB-CLONE-3183796
Clone Name: IMAGE:7851974
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Gene:
capn11
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391955 [+]
ataactcctcagaatggaatgaagtggacccttcagagcaggaggatctgcagttaaagatggaggatggagagttctggatgtctttccaggaattcct
gcgccagttttcccggctggagatctgtaacctgacgccggacgcgctggacaaggacgggcagagcaagtggcacagcaccctgtacgacgggacgtgg
cgccggggcagcaccgccgggggctgcaggaacaacccagccacattctggattaacccccagttcaaggtgaccctgctggaggaggacgatgaccccg
atgacaacgagttggcctgcagtttcctggtggcgctgatgcagaagaaccggcggaaggagcgcaaggtcggccaggacatgcacaccattggctttgc
tgtgtatgaggttccggatgagttccgggggtgccagaatgtgcatctgaagaaggatttcttcctgcgccaccagtcctgcgcccgctccgaaaccttc
attaacctgcgggaggtgagcacccagatccgcctgccccccggcgagtacatcatcgtgccctccaccttcgagccccacaaggaggcagattttgtcc
tgagaatattcactgagaaacattcagatatacaggagctggatgaggaggtatcggcagatcttcctgaggaggaggaactcacagaagacgatgtgga
cgattcgtttaaggccatgttccgccaactggcaggngaggacatggagatcagtgtgtttgagctgaagacgatcctgaacagagtggtggccaaacac
aaagatctgaagacggacggtttcgggatggaggcctgcaggcagatggtcaatctgatggacaaagacggcagcggcaaactgggcgtggtcgagtttc
agatcctgtggaacaa
3' EST: DT391954 [+]cagttttcattctttattccatgcagtctggcacaattccgcctctagggggcgacagtacatttgcactcagggaaacctcaaacataactgccccact
ctgaaagcttgtggttctggcccggggacccctgtaagagtcagatgcccccccagtacaagaaggggccccggtatctgtatatacaataacaaaacgc
tactgggcggagccctggggccggagtacagtccttacagttgtgcctggcagctgcccgcttggcattggatacattatacgggcagcattctgattgg
ctggttctgattgcaaaatctttcccccaacaaaggcctctgggaaatgtgtaacctggatgtaacgttggtagcgagtgagcgagggcaccgggggcaa
tgagtgtcagcgagggggcaagagaattatccacacatggtcatcgtcagccactcccctaaactcatctcggctgtgccctccccttggtccaacgcct
tgaagaaccggaacatggcctccagcttcacgaggcagcagacgaagttatcgaagtcgattcccatgtcggaatccgcgtagcgagcgaccagaacctg
aatgagtttgttattcagtttgtaccctgtaccctccagggcgatgcgcagctcgtaggcgctgatggtgcccgacttgtccaggtcgtgttgtcggaaa
gctgtcagccattggcgaattttgttccacaggatctgaaactcgaccacgcccagtttgccgctgccgtctttgtccatcagattgaccatctgcctgc
aggcctccatcccgaaaccgtccgtcttcagatctttgtgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: