XB-CLONE-3183779
Clone Name: IMAGE:7852262
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391966 [+]
aaaacatgctcttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataatgacgaagtg
aagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctattctaccaca
cacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtgatgttactt
taaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaaagttttgtc
tctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacccaatattcacagtaat
tcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctgaggtttgc
ttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttccagtttcatt
ttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatttccctatt
caaataggaatatgtgatagcgtaaatcttttcgactttgtcttatacaggtaggtacaaaaatagcttttatatttacaaatcagttcatctagttcta
gtgaatcacttgtaaaaatacatgtgaatgtaaacgat
3' EST: DT391965 [+]tttaaggtttgccctgtgaacatcagtgtagttctggacacacnctttaaaagtgtcttttcccaagtgtccagtgcaattgagtcaaagaggataattc
agaaatctaaatccgaagtttcatctcaatcaaaaatgattatggaggaaaccagcaagactgaactggtcgctgaagaaatcagagctactcaggctga
aattaaagctttagtaagttctgtaaaattcagtgaaggccaaaaagtcacactgaaagccaatatacctggagcatctcaaataaagtggattctaaat
ggatatgaagtcagcaactctgaaagctacaggtatggagtgtcgggcaatgaccagactttaactattaagaaagtcagtgctaaagatcaaggaattc
ttacctgtgaagctaaaacagaagccggacttgtcaaatgtcagtttgacctgactgtgagccaagatgtttcaaatgcaccagccttcgtctaccaacc
taagtctcaaaatgtgaatgatggtcaaaatgtcgtatttacatgtgaagtcactggtgatccatctccagaagtcgagtggtttaaagacaatatacct
gtttctttgtcctcccatataagagtttcacgttcaaaacaggtctacactcttgaaataatccacgcaacagagaaagatattggaaaatacacaatta
aagcaaagaacttccatggacaatgctctgccacagcatccttaaatgtgcatcatcttatggaagaacctgtcaagcaggttgtattacagacagctgg
ggaagtcagccttcaggaaggattctccttccaggcaatgaaaatgtcttcttccccaggcagagcctccttcagcagtatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: