XB-CLONE-3183546
Clone Name: IMAGE:7852092
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392121 [+]
ggaaatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttca
ccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgtt
caaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggt
gatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtc
gtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagca
gcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctgg
cacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagac
gcttctgtgagggacttcagaagattgaagagatctttaaacaagcaggacaccccttcatgtggaatgaacatctgggttatgtccttacctgcccatc
anacttgggcacaggcctg
3' EST: DT392120 [+]agaggctttttttagaggtgatattttggttttattgtctccaggcaatcatgggngggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataa
tataatctttatcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagatta
tcccacccacatccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaag
ggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatct
caatcatgagtttcacaccatccactaccatctggacttggtcaacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgc
tgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatgg
acacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatct
cttcaatcttctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttac
ccacaccaggaaggtcttgttttcattgtgccagataccacgggcatcaggccagtca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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