XB-CLONE-3183476
Clone Name: IMAGE:7852194
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Gene:
arrdc4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392167 [+]
tgaaatgaaccagatgtctagcaaaggtgatgctgctctttttacctgtatctgtgatttattttcaacttgaagtcttacagaaagaccacagaatgtt
tgcacagatcatatatagaaggcaaatgtgatgccaactgcttgtatgcagatgaaattgctgaccgcccgagatcaacagatgatatttgcagatcagc
actttctgcagaagacaaagggaattccatgcctttaaaagggaacacggaattttaagggaattctacaaactatttgatgtggcgcattgattggtgc
tcgctaccatttcctacagtatttgtaaatataaaagaaaatgtgagctttatgcaagttggtagtgatacaactacataatactgccaaaggtcctgtt
gcacatgatcagtctttgtcttgctttgttaaatttgtcttactcctaataagaaagaatagagaaatctagaaggacagtttgagagaagcagagggac
aaacaggagagaaagagaaggaagcctggactagagggagaggtagaggaacattagatagagcagcagaaggactgataagagagagagagagaggtgg
attaataagccagagctcatgagagaagaaaagggactggctagacagaaaggatgcctcacagagagattatagaaagaggtggaaaggcaaaataaag
aacagtggggagtatgtaaagaacggttaccctaacggctcttcaggttgagacacagacaaaaagtgagagagtatactaattataactaatattttct
ttgtggttttctttcctctantactcacactatgggaactttcatttaactcttaatgcagacgttttgattttgaagagatactctagtttttgctgcc
tgtattatgtggcatttattgtttgtggc
3' EST: DT392166 [+]gaagtaaatctctttatttatgataaaacaagaacccacaggattcatataagttacaaacaccatcttgtttttttgttatcaaaacagaggcttgtaa
gagaaatgtgcaaatagaaatgcatagggtttaacaacaagatatcaaagaggtacatttcgtacatttataaaacaaaacatcccatttaatggggaaa
tgtaaggccacgatttgatagagattacagcaggcatatgtatgtagatatgtaaaaataataacatggttactatccaacaggtctctttaaagagaat
gcttgctattatataaaagccgatacagtgcatgtttaatattattttacagcagtctaataatgcggaaattcctcagcctaagttattttggactaca
cgtatgataatcccagcataagtattgcagctactgatgtactggctgtactgtctgcactaggacacaatgttaggctgaagaatgctaattaatgttt
gtatctcacaaacgatatggaaacaaatacttgtgaaatatatatatatccccccagtttagatgcatgcttatattaatgacagctctcctttaatccc
tgcaggaccacacaatgtatatattagtggaatacatgtctgtatgaaaggcaccctacattttgctttgtatcccaaataaatcagtttcataagatgc
actgatatcatacgcaagctagctatttcacttttaaccagcttggatttggagtaaatattaataaagactcatagaaggacggaatcgttacagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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