XB-CLONE-3183473
Clone Name: IMAGE:7852242
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Gene:
sorbs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392169 [+]
gctggtttgtaggtacttctagaagaaccaaactctttggtacttttccaggcaattacgtaaaactagtattttattaaagaagatggtgttcagaatt
gctgggtaacatccatccggaggcttgcttgtgggaatgatgctaaccaaaagcctttgctgaatgtattagataaagttatttatagaggtatatggtt
tacacatgtcagttctgggtttaagaagtggccctctgattcctttatatatgatgaagttaagacatatttcttaaaatttatattgaacttgttgcct
tggaaacattttatttaagttctaaatttaaatatcacttttatttttatttcaaacattttaatcaaaacgaggaaagacatatttttttatattcatt
tcagctgttacatttgtatatttaacagcctaaatgagacagtgagaggcaacagagaatagttatttttcaggaaaacaagataagccccctgagctaa
atacattggttcgtgtatgcccttctacagcccctcagctgtgttggggtataaccctccccccccccttagattgaagctgttttatttcagtagtagc
agaagagtgcagacattggtcagccagctcttaccaactgttataaactaattactgagcaatcaaaattgatgtgattttttttttacatggcaggaaa
aatgatatggtacaatatatgacattgtaatcgtctataggaaattctgtgtactatagataacattcatgcacaanaccatggggaaaaaactattttc
aggcattgttcataatggtgctctaaatgctcaaatgcgccatcagtgttaatgggagtccgttgccctttatgctatctgaacagttataggggtga
3' EST: DT392168 [+]tttattcaatgctttaatttattaggcttcaaaatataatgcactgcaatatgaaaacaacacaacataacttgaaaagtgactggtatggtgataactg
aacaatgctgttaaagtgtattggattatacggttgggtaaggaaagcattattgtgaattagccaaatggtaacataactctaaatatggtggctaaaa
attctgttgaaagaaaaaaacattaataaaacaatggatatgtttgctaaaaggtgttcagtgtcatttcctcatgtgtccaatgattctgtgcttaact
tttccttgtgtcgttaacaaaatcgttcattaaattaaattcaagacttcgttttcctgaattttatgcttttctctatccatcagtccacttaaattac
tctttttttctatacaagatacaacatacatgttctttacaaagtcaaatatacatatttacagtttgtctcttatgaaccatttgtaatgtggatgtac
tgtgtacatggaaaaagaaaaaaaaaaactttaataccagtaacatgggtaatatatcaaaacatattgctagtcatgccaacgcagctgcaattgttaa
taaatctgtataaaagcaatagcaaaaaaatagtcatcttatattaaatatatcttgttatgacaaaagaacattctcaatctttttttttttttttttc
ttttatgaccataagctataaacttacaatgttgccctcactttttttttttttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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