XB-CLONE-3183455
Clone Name: IMAGE:7852148
|
Gene:
spon2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT392181 [+]
aaaaccaacaggtgaagtgacatgttatcacaatgctgttgaaatccaggagagtagcaagattcatataagtaatgtaagtggtgtcgtaacgctagaa
attattgattgccagccagaagacagtggaacataccgtgtagtgtgctcaaactacaaaggggaaacctctgactatgcaacattagacattgccggag
gagatttttcatcatatgtgcctaagcgcagagatgatattgttcctaagtcagcctttcctgaattattaaagtcagaagactatgccgtctcatcttt
caagaaaacttccgcaatggaagctagctctgcaagaagagaggttagttcacaagtaacatctttaagagaatctagagagtcatatgaggcttatgca
tctgaagagaagcgagtttctgcctctgaagccaaagctgtggaagaaagagttgttcacaggaagtttaagtcttcagagccagcaaaactactgacaa
ggccacagtcaatcacagttgctgaaggtgacagtgctagattttcatgtgactttgatggtgagccagctccaacagttacatgggcacatgctggaca
aacaatcttttcttctgtgcgtcatcaagtgacatcaacaaaatacaagtcaacatttgaaattacctctgtcaactcttttgatgaaggcagctacaca
gtagtagttgagaattctgaaggaaagcaagaagcacattttactttgacaattaaacgtggaaaggcagcagaaaagggtgttacatccccatctagag
taaaatctccagagccacacaaaaaatcccatgagtctataaagtcaccaaaaagagtgaaat
3' EST: DT392180 [+]ggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataatgac
gaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctattct
accacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtgatg
ttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaaagt
tttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatattcac
agtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctgag
gtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttccagt
ttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatttc
cctattcaaataggaatatgtgatagcgtaaatcttttcgactttgtcttatacaggtaggtacaaaaatagcttttatatttacaaatcagttcatcta
gttctag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: