XB-CLONE-3183125
Clone Name: IMAGE:7852369
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392399 [+]
atcgattcgggaattcttagagagattcgatatgcgtctggagcgaccttcgttgcagcgcggctcaatatcctgcgactaacgaagatgtaacgacgcg
ccaggcaatggaagcttaggccaaggatatagagaacatgaacgaactatggggcttactgatggtgggacatgttgctgggtcaaaatattcctaaggc
cccacccctgaggagcaaaaggcattcctgcctaacattttcctgtgctctagaaaggcacaacctcggcccaggagctgacaattccttacacagtttg
tagctatcaactccctgtcatttctacgtgtgcggccacaatgcccaacagtagccatcctagccacagaggcagttcaagctgatattatggtacatat
acccacagattatatcattggtagggatgcactgaatccaggatcaggttcagcaggatttggccaaatccaagtgtctggccgatttgggttcagttca
gtattcggatgaatctttcacaaagaatttgttgttcggtcaaaccccaaaataatggatttggtgcatccctatttgtaggaaaccaaagccccatctc
aacatatgcaaccattcctcacctgaggcagcattttacccccaaaaaatccttacaaccccaatcggagctctcctagactggtctgaatcacacccag
ttgctaaatgattttcaaaaaattgcctgcagtccagtaaactggtctggtacaattccagcaatgaaaatgagcaattggttgccatgggttactggac
cagggcaggtacctaaat
3' EST: DT392398 [+]cccagaaagcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatat
aaaaatccttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggc
tgacctttattgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggg
gaatttaggtacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaatt
ttttgaaaatcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggt
gaggaatggttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttg
tgaaagattcatccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctac
caatgatataatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtagaaa
tgaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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