XB-CLONE-3183104
Clone Name: IMAGE:7852323
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Gene:
etf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392413 [+]
tttacgtcacatctgggagcctgggcgacttcatttagatcttttctgggcagaatcgctgcgctgcggacaggagcagcgagggtggagagaagatggc
ggatgatccgagtgccgcagataggaacgtggagatatggaagatcaagaaactaattaaaagcttggaagccgctagaggcaatggtaccagtatgata
tctttaatcattccacccaaagaccagatttctcgagtggcaaaaatgctggctgatgaatttggtaccgcatctaacattaaatcacgagtaaacagac
tttcggtcttgggagcaattacatctgtgcagcagagacttaaactctataacaaagttccccctaatggtctggttgtttactgtggaacaattgtaac
agaggaaggaaaggaaaagaaagtcaacattgattttgaacctttcaaaccaatcaatacctctctctatttatgtgataacaaatttcatacagaggca
ctcacagctcttctttcagatgacagcaagtttggatttattgttattgatgggagtggagcgctttttggcacccttcaaggaaacacgcgagaagttt
tgcacaaattcactgtggatctgccaaagaagcatggtagaggaggacaatctgccttgcgttttgcccgactgagaatggaaaaacgacacaactatgt
ccgtaaagttgctgagacagcggtacagctgttcatctccggcgataaagtgaatgtagcaggtcttgttttggctggatccgcagactttaanactgag
ctcagccagtctgatatgttcgatcagcgattgcagtccaaagtattgaagctcgt
3' EST: DT392412 [+]tttttttaggtctgcatatatattttttttaataacctctcaagttttcattcttcacatgacatttcttgtgcaaaacagaatgcagtttatcagggtc
atttagtaaatctgttccctctagtaatatgaataaaaggaaaccgagacattgtggtgtttcagttacatgtatgtctgcgtcgtgtacggtttttccc
atagtataaagttaaaaaaaaaaatcttaaaatacaggaacaaataattttcgaaataaaagtaaaaacggatctggtgagagtagagcaaagtgttatt
cggggaactttgcgtttgcactggacaatatgacagagcaaagttttctttttcttttttttaatccaacaactagaaatgttccaattctaaggcaggg
atctgatttgtttggcttccactatgaaacaagtttcttgggctggatgctggagaactaggcacgtctttgccagacccacttcaactacctagtagtc
atccaggtcgaaaaattcatcatcgcctccttggtactccattccctggaagtctactctgtatcgtaagattcctccaattccaccaaaccctttcaca
aactgagagccttcttgtgacttatctgtaacaatctccagtgttgctccaaatttcttgtagttattagcaaaccactctagtaaaggcatactctcta
ttaactcatgctcttgtccagtttctttgtcaatgaagtgagacttgtccttttcttgctcaggagttaggtaaagagtcttctcttcttctgacccatt
gcaccttaagacgtatctcatgatatccaagttctcgtatactattagaatttccaccgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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