XB-CLONE-3182989
Clone Name: IMAGE:7852429
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Gene:
slc5a8
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392489 [+]
ccgaggacaccatgtccggagacgagatgattttcgacccaactatgaccagaaagaaaaagaagaagaagaagcctttcatgctggatgaagatggggc
tgagccacaggcagaagagacgcaggtaccggagactaaagaagtggaaccagaaccaccagaagataaggattttgatgtcgatgatgaggcctttaaa
aagaaagtcgatgcccctgatgacctggatgatttgaatttcttgaaccagaagaagaaaaagaagaaaacaaaaaagtttgacttggatgaagcagagg
aaggggttaagaatcttaaaattgagggggagattcaggaggcatctgaatcccaggaggatgatttggatttcatgctgagcaagaaaaagaaaaagaa
aaatgtgaaattcccagaggaggacgataccctggatagagatgaagccttcgaagaagaagacaataagaaagatgatggaatctcgttcagctcccag
ctgggtccggcctgggccggcacagaaagggactatacatacgacgagctgttaaacagagtgtttaatatcatgcgagagaagaatcccgacatggtgg
ctggggagaagaggaagtttgtcatgaagcctcctcaggtcgtcagagtagggacaaagaaaacatcatttgtcaactttaccgatatctgcaaactgtt
acatcgtcagccaaaacacttgctggccttcttngtagctgaattgngtacaagtggctctatagatggtaacaaccagttagtcatcanaggcagattc
cagcagaagcaaatagaaaacgttctaagaaagatatatcagggatatgtgacttgtcacacctgccgatcgc
3' EST: DT392488 [+]gtgaatcatgtaataactttaatgtatttttgcattttacagaggtttggttaaaaatgcatacactaccttcccacttcccctcaggcagctggaaaaa
ttgttttccgagagcaatgcgagtatatggaataatcggctccattgtctgctttagaatctctttgcgcgggcaaagatttaaaaaaaacaaaaaaaaa
attagccaatgtgttttaggcctcttctcacagcattccttataatgccagggtactgcgccccctgtcagcaagtccctgcaatatatcctcgcaccag
gctggcaaaatcccaaatggtttggaagccagttagttggctttggcacggagctgtgctctcttgcccgtgacggcctggaatccggttttgatgctgg
ccacggaacaccgcgagtggcaggtttcgcactgcaggaaatataatcgggtgtccttctgcaagatggtgtctggcgatcggcaggtgtgacaagtcac
atattccttgatatatcttcttagaacgttttctatttgcttctgctggaatctgcctttgatgactaactggttgttaccatctatagagccacttgta
cccaattcagctaacaagaaggccagcaagtgttttggctgacgatgtaacagtttgcagatatcggtaaagttgacaaatgatgttttctttgtcccta
ctctgacgacctgaggaggcttcatgacaaacttcctcttctccccagccaccatgtcgggattcttctctcgcatgatattaaacactctgtttaacag
ctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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