XB-CLONE-3182892
Clone Name: IMAGE:7852677
|
Gene:
mlf1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT392553 [+]
acaaagtatttattttcattggaaacattaaatcacaattaagttttgattgttgcacaatacagaatctacatcattgtataaaattagttccagcata
tttactatgtagaacacaatgactattcatgatttaaaaaaattacaatttataatattactttaattgaattattttcactagaccactaaagcaaaca
cagctattatacaagcctctgcaactaatatagttaatgggggcattaactgagataaatatttaaccagtttaattagaaatctttttacaaataattg
tacacattaccttacaaacatgctgtgctagcttatctttccatgaactgtgcaatatatatatttctctctctctctatatatatatataaatacacac
ttccacacattttctttgcatcatagccacctattaatgtcaatgcccaaggcgttactgctacaaaatctatgcaatttcttattatgtagatatagat
gtctacagagtggtgagacacctccaacataagagtccagaacagatagctcatccaaaaaatgcagaggaaactcatgtgcatgtctcaatcatttatt
tagtgatctccttgcacaagatggtttcacaccattggctggaacacttcagcttttaggtgactggtgccgagtctcacttctcccttgctggcaactg
tggctggcttttgcttggtatatgtttaacagttctgtgttttggagcttctactgcatttcctggaaatgacttaatctttaaaacctctctttgccat
tcattatcaa
3' EST: DT392552 [+]tttggacgtgatccatttcttagtatcacagatggccgatctcggggtcggagagggcagccagggtcccaggtggctctgagagaagatcacagggctt
ccagcctcagctctatgcctactgacagttttactggaatgagttcagacttcagagatgttttccactccatggatagtatgatggcaaacatgcgcaa
cagaatgagagatatgcacagaaatgttgaaggagatccttgtactcactcatttagctcatcatcagtaatgacatattccaaaacaggagatgaacca
gcaaaggttttccaagcttcaagtcagacacggagagctccgggaggcataaaagagactaagaaagctgtaagagattctgaaagtggaattgagaaaa
tggctattggtcatcacatccatgacagagcccacattatccagaaaagtaggaatcacaaaacaggggatcaagaactcaaccaagaattcctcaattt
ggatgaagctgaagggccatcttttgataatgaatggcaaagagaggttttaaagattaagtcatttccaggaaatgcagtagaagctccaaaacacaga
actgttaaacatataccaagcaaaagccagccacagttgccagcaagggagaagtgagactcggcaccagtcacctaaaagctgaagtgttccagccaat
ggtgtgaaaccatcttgtgcaaggagatcactaaataaatgattgagacatgcacatgagtttcctctgcattttttggatgagctatctgttctggnac
tctatgttggaggtgtctcaccactctgtagacatctatatctacata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: