XB-CLONE-3182691
Clone Name: IMAGE:7852314
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Gene:
lrrfip1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392685 [+]
cattaaaatgttccatatttgttatattatttatttgaattccttgtgtggcgtcttttacctaggaaagcattttcttttgatgttatgctcatttgct
aaaaaaaatttagagctctttatgagaaagaggtcagcgcagtttaggtagtattagtcaagctgactaaacataaagagaaccacttctgtggccaagt
ccaggagtaggcaaaacctgccttgatcaatgcattcaaatctggggacatgatcagatcacacaagagactgggtgcaggtggctacggactgaaatat
tggctgataatatgctaatttcaactaatctgcagttaaaacttgtatatctacggttaattttaccataaaacagctccaaacaatattcacatggaga
acagaccaggtgacagttcaatttgattatccatgtgttccaaaactaatgactgcccaacaagccagaggattggatctctcaaaacagtttctatgtt
tatattatttataattaaagatagccgacccaaacctgaagactttataacctccatctttctgcaattatttccactttctatatagcacataatttag
tgctttctctgccactttccttctccttctccagtttactaagaattatgtggattatctagtctacggtaggtacatggaggtctcaagggaattccaa
caaatgttggtattacatgtttccttccag
3' EST: DT392684 [+]ctgagccagggtacctaagatctgagcggatgcattgtaacaaggagagggaggtacatatatttactgcagcagtagatactccataacctaaacgtta
gggaccaggtggccaggcagcagggaaagaaagaaaataccccactaccagtcataccgctgggcacttacaaagctcagagcagcagagatagatacat
acccggagcagcaagtcctatctaggtgggcgtagaggattctgtttcaccaccatcccttcatctctaagacaagcagtgggagtctctctgtgggagt
ctggatgaggatacagggctaagaaaccccctttcactcaccattcactactctgggagaagatgggcacccaaggacctggaaggaaaagacaccccaa
tcaggagaggctttctgctgaggatcaagccctgaatcagattgcacgagaggcagaggcacgactggccgccaagagagcggcgcgagctgaagcccgg
gagatccgcatgaaagagctggagaggcagcaaaaagagatctaccaggttcagaagaaatattatggtttggatgccaagtggggagacattgaacagt
ggatgggtgcagatgacgatgaccttatgtctgtgggaagtaggagcagtctgaggaccaatggttatgaggatgaattacttggagcaactcaatatag
gaagtccagcaggagcacttactactcagatatgagcttggccagcgcttcactgacctccaaacctcagcaggcaatgcacaatggaaaccggccttct
ctgctcaactgcaacactctcccttccagatctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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