XB-CLONE-3182551
Clone Name: IMAGE:7852566
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Gene:
mmab
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT392778 [+]
ccgaggggtaccaagagctttgctgggatattcagcggggaggcgactgtatagtgaaaagtgtacgaaggagcgcatccaggatggacccaagaccaca
aaggttccaaagatctatacaaaaacaggagacaaaggtttctccagtacatacaccggagaaaggaggcccaaagatgatctgctgtttgaagcactgg
gagatacagatgaactgaactctgctattgggcttgcaagagaatttagtctagatgccaatcacatatttgtgcctgaacttgaaaagatacagtgtat
gcttcaggatgttggatcaaacattgcaactccactgtcttctgctagagaaagccacaaagtaagaacatcattcagtgcagaacctgttcaggagctt
gagcaatggatcgataaatacacctctcaacttcctccactgactaacttcattttgccttctggaggtaaaaccagtgccgcccttcatgttgcccgag
cagtgtgtcgcagagcagaaaggaaagtatttaaagtcattcaggctggagaggcagatcaaaatgttggaaaatacttaaacaggctgagcgattgtct
attcacattagcgaggtatgccgcacaagcagaaggcaggaaagagacagtctatacaagaacaccctgaagcttccatacatgtgtaacatcccgaatg
tgcaaattgcattgtatttcaagtttccttgtggtagctgaaaggccggctgagtcagccatatggacagtttgacgttgctaatggtttgggctcctac
agtacattcaaggcctgctttaggtattttcaggcattctctga
3' EST: DT392777 [+]ctaatcatgcagtgtttattgattaaggcccagtaaaatataccatgctgtaataaatctgcccaatttaccaatatcaagcatttacacttctacggtc
accttgccaacatcatgtcttcattgttttaggtttccattaaagtgaattcatctcctgtactaaatcatgctttatttcttcctcacagtctgacaca
aacctgtacaggttaacttgaaggtttctttttgtgtgatgagttcacttaaccaagatgatagtagtattcctgctttggcagctatcacagtttcaca
gcagtggatgtagaaggtattcagagaatgcctgaaaatacctaaagcaggccttgaatgtactgtaggagcccaaaccattagcaacgtcaaactgtcc
atatggctgactcagccggcctttcagctaccacaaggaaacttgaaatacaatgcaatttgcacattcgggatgttacacatgtatggaagcttcaggg
tgttcttgtatagactgtctctttcctgccttctgcttgtgcggcatacctcgctaatgtgaatagacaatcgctcagcctgtttaagtattttccaaca
ttttgatctgcctctccagcctgaatgactttaaatactttcctttctgctctgcgacacactgctcgggcaacatgaagggcggcactggttttacctc
cagaaggcaaaatgaagttagtcagtggaggaagttgagaggtgtatttatcgatccattgctcaagctcctgaaacagttctgcactgaatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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