XB-CLONE-3182512
Clone Name: IMAGE:7852426
|
Gene:
spon2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC122078 [+]
ctttgctgccacaaagcaaattaaagtgaaaaacatgtttctggtctacaaacattgcactgtggtcctagccatgctatcgatggtggagtattccagc
tgccttcccttgagtgaagattctatttgtaccgcagaggaaattgctaagtatagcatcgtttttacagggaagtggacccaggcttctttccctaagc
aataccccctttacaggcctcctgctcagtggtcaacgttgctaggagtcactcacagctctgactaccacatgtggaaaaaactggagcctgccagtaa
tggagtgagggactttgcagagaaaggtgaagcgtggccgctaatgaaagaaatagaaacagccggggaaaaaattcaaagtgtccatggaatcttttct
acagcttcaatctctggtggcacaggacaggcctctactgagtttgaggcacattcaaggcacccctttgtctccttcatggtgagaattgtaccaagtc
ccgattggtttgtgggcgttgacactttaaacctttgtgaaggaaagcactggaagcaaacagctacattagaacttcatccgtatgatgccggaacaga
tagtggatttacattttcatcaccaaattttgctactataccacagggcattgtaaccgagatcacagcttcatcaccaagtcatcctgcaaactcattt
tattatccaagactaaagagcctgccacccatcgccaaagtcgtatttactaaagtgaaaggacggctttcgtcatttctcgatattgtttctaatgtca
ctacaacagggaacgagatagctgaacatattttagaaactccactggattgtgaagtctctgtatggtcatcttggggtctctgtagaggcagctgtgg
gaccgctggagttaaaagtagaacaagatacattcgcctgaagcctgctaacaatggaattgcatgcccagccctcaatgaagataaagaatgtgatcca
gagaattgtgtatagaacaacaaatgactgctaaataataacttattttagatatatggtacatttacttgtaaataacagttcaggacaacatatgata
tggtttaacgaacgtaatgcaccccaaccagtggcttgcaggcaacatgttgctcatcgcccctttgatgttgctcccagtggcctaaaagtaggtacca
ttttcaaatctctggcttggaggcaagttttggaagcccagagacactgttttattccaagcagagcctccaggccagcagtcacattgggctaccaaat
agccaatcacagcccttatttggcacccctaaagaacatttttcatgcttgtgtgactccccaacactttttacatctgagtgtggctcacaagtaaaaa
gggctggggatccctgatctaactgatggttcggacaacatttcacagagaaaatgattttttttctggaggctggagctcgattccaggttttcttgtt
tcatataatattcctttgtgggacaataacagttttattttttttacacattacacttaagatgaacttaagcgaaaatcattcattttactatcattct
gtatttgtatatacatattcatgctcacaatattgagcaaatgttcataataaagagttctaagttttttctggcaaaaaaaggaaattgatacattttt
aactgacatgtacatactgtatatagccgagtttaccggttatggagttctgtaccatatattttatgacgttttaaaatgaaactggaaccaacggtgg
gcaggagaaagtttcctagggagcgaataccttgcctgccattaaactatgtgcctcaactctacgttgatgtctaagcaaacctcagcaggttgagaaa
ctccctgttgaagacaaacctacacccaagagaggatgctctacacgaactatacgaaatccaagtatttggattgaattactgtgaatattaggtgctg
cttgcaactaccctcctgcaggcaacacctacatatttacattatttacagataatggaaatttacaattttacagagacaaaacttttgtatgttcaaa
gtgcaagcggccagcactttgttcataaaaaaccctctgcactttttctgacacattgactccctcagcttgctttaaagtaacatcacctggccatacc
ctcaacaaagcaggttaaggtactcaagtgcagaatcagtgccaacttcaggaggtttatcgggtgcagtgattgtgtgtggtagaatagggactatatt
atatgttgagaaaacagggcaataaagtgatgcaccgcagcttttcaggctgttgttaagaacttccgaatgcccttcacttcgtcattatttattgttt
tattttatgttattgtagcattattatttattgaacctcccgaagatagctctttgattgcaataaagcatgttttctgcaccataaaaaaaaaaaaaaa
aaa
5' EST: DT392804 [+]ctttgctgccacaaagcaaattaaagtgaaaaacatgtttctggtctacaaacattgcactgtggtcctagccatgctatcgatggtggagtattccagc
tgccttcccttgagtgaagattctatttgtaccgcagaggaaattgctaagtatagcatcgtttttacagggaagtggacccaggcttctttccctaagc
aataccccctttacaggcctcctgctcagtggtcaacgttgctaggagtcactcacagctctgactaccacatgtggaaaaaactggagcctgccagtaa
tggagtgagggactttgcagagaaaggtgaagcgtggccgctaatgaaagaaatagaaacagccggggaaaaaattcaaagtgtccatggaatcttttct
acagcttcaatctctggtggcacaggacaggcctctactgagtttgaggcacattcaaggcacccctttgtctccttcatggtgagaattgtaccaagtc
ccgattggtttgtgggcgttgacactttaaacctttgtgaaggaaagcactggaagcaaacagctacattagaacttcatccgtatgatgccggaacaga
tagtggatttacattttcatcaccaaattttgctactataccacagggcattgtaaccgagatcacagcttcatcaccaagtcatcctgcaaactcattt
tattatccaagactaaagagcctgccacccatcgccaaagtcgtatttactaaagtgaaaggacggctttcgtcatttctcgatattgtttctaatgtca
ctacaacagggaacgagatagctgaacatattttagaaactccactggattgtgaagtctctgtatggtcatcttggggtctctgtagag
3' EST: DT392803 [+]atggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataatg
acgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctatt
ctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtga
tgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaaa
gttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatattc
acagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctg
aggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttcca
gtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatt
tcct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: