XB-CLONE-3182220
Clone Name: IMAGE:7852931
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Gene:
acbd4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121676 [+]
GAACTGCAGCTATTATAGAATATTCTCCAATTCAAGGATAGCGCAGAGTTCAAGTTTTATCAAGATAACTCAGATACAGCGGTGACAGCCGGTGCCCCAG
AGCTGTTCAGCCAGAATTTCATACAGTTTACTGTGTGCAGGTGTCCCAGACCATACACCAGAGAGATGGGCACAGACCAGGAGGAAACAGGGTACCAGAA
ACAGTTTAATGCAGCAGTGTCAGTCATACAGAATCTGCCTAAAAATGGCTCATATCGTCCATCTTACGAAGAGATGCTGCGGTTCTACAGCTATTACAAA
CAGGCCACGGTGGGACCCTGTAATATCGCACGTCCTGGTTTCTGGGATCCTATTGGCAAATACAAATGGGATGCTTGGAACAGACTGGGCAGAATGAGTC
AAGAGGATGCCATGTGCTCATACATCCAAGAGATGAAGCTGGTAGCACAAAAGGTTATAGACACTGTGCCACTAGAAGATACCTCTCCTGAGATGTTTGA
ACATTTCCGCCCCCTATATGAAGTGATCCCTGACATGCCACGCCCCCCGGATTCCTTTTTCAGCATGTCTTTTGAAGAAACAACTTTCAGGGAAGCAGAG
GAGAAACAGCAGGAGGAGGGGAATGAAGAGCTGTCATATTCACTGACACAGAACTCATTCAGCGGCGGAACAAATGTTTCTCAAGCAGGAGAAAGTCAGA
GCATTTCCACTGCCAGAGAGAGACGAGGAGATCCTGTAGGAGAGGATTCTCACCAAGCCCAAGGTGAAGAAAGAAGAGGGCAGATCTTGGGAGCAGAGAC
AGAAGACTCCAGTGACAGCCTGGAACAGTTAGATCTTGATAAGGACTTTAATGATACAGTTACATGGATATACCCTCACCTATTTCGGCCATCCAGTGAT
GTAATACCCGCAGATTCAAATTTGCAAAGGCAGAAGGAAGACACACTGAAGGTCTCGGACCATGGCTGTGAGAGAGATGCCTGGCAGATGAACCCCAGTA
GGGAGCACAACTTAGACAGTGCTAAACGATCACAGAGCAGGTGGCGGAAGTCCCAACACCAGCTGAACCCCCAGATTATGGCTACGGTGCAGTCCCTGCA
GAATTCCATCCAGGTTTTATCTGATCGTTTGGAAAGCATGGAGAAAGCACTGAAGGAACAACAGAGGGTTGAATGTTCACGTCGATACAAACCACAGAAA
CCCTGGTTCTCATTCCCTGCTCCGTCCCGAACTCTGTTCTTCATACTTGTTTGGCCGTTCATTGTGAATTGGCTTTTGCACAGATATTATAGAAGAAAGA
GATGACCCCTCCCTTCACCCCGTCCATTAAAAGCACCTATTTCTTCTCACCTTTAAAAAAAAAAAAAAAAAA
5' EST: DT392998 [+]aactgcagctattatagaatattctccaattcaaggatagcgcagagttcaagttttatcaagataactcagatacagcggtgacagccggtgccccaga
gctgttcagccagaatttcatacagtttactgtgtgcaggtgtcccagaccatacaccagagagatgggcacagaccaggaggaaacagggtaccagaaa
cagtttaatgcagcagtgtcagtcatacagaatctgcctaaaaatggctcatatcgtccatcttacgaagagatgctgcggttctacagctattacaaac
aggccacggtgggaccctgtaatatcgcacgtcctggtttctgggatcctattggcaaatacaaatgggatgcttggaacagactgggcagaatgagtca
agaggatgccatgtgctcatacatccaagagatgaagctggtagcacaaaaggttatagacactgtgccactagaagatacctctcctgagatgtttgaa
catttccgccccctatatgaagtgatccctgacatgccacgccccccggattcctttttcagcatgtcttttgaagaaacaactttcagggaagcagagg
agaaacagcaggaggaggggaatgaagagctgtcatattcactgacacagaactcattcagcggcggaacaaatgtttctcaagcaggagaaagtcagag
catttccactgccagagagagacgaggagatcctgtaggagaggattctcaccaagcccaaggtgaagaaagaagagggcagatcttgngagcagagaca
gaagactccagtgacagcctggaacagttagatcttgataaggacttttatgatacagntacatggatataccctcacctatttc
3' EST: DT392997 [+]aaaggtgagaagaaataggtgcttttaatggacggggtgaagggaggggtcatctctttcttctataatatctgtgcaaaagccaattcacaatgaacgg
ccaaacaagtatgaagaacagagttcgggacggagcagggaatgagaaccagggtttctgtggtttgtatcgacgtgaacattcaaccctctgttgttcc
ttcagtgctttctccatgctttccaaacgatcagataaaacctggatggaattctgcagggactgcaccgtagccataatctgggggttcagctggtgtt
gggacttccgccacctgctctgtgatcgtttagcactgtctaagttgtgctccctactggggttcatctgccaggcatctctctcacagccatggtccga
gaccttcagtgtgtcttccttctgcctttgcaaatttgaatctgcgggtattacatcactggatggccgaaataggtgagggtatatccatgtaactgta
tcattaaagtccttatcaagatctaactgttccaggctgtcactggagtcttctgtctctgctcccaagatctgccctcttctttcttcaccttgggctt
ggtgagaatcctctcctacaggatctcctcgtctctctctggcagtggaaatgctctgactttctcctgcttgagaaacatttgttccgccgctgaatga
gttctgtgtcagtgaatatgacagctctttcatccc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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