XB-CLONE-3182127
Clone Name: IMAGE:7852939
|
Gene:
srrm1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT393059 [+]
caaagaaggagactgaaagcgatggcgaaggaaatctagacgatttggagaagcatttacgagagaaggctctgcgttcgatgagaaaagcgcaggtttc
tccacattcctagttgtcacttcagtttgctgccatgtaaattttttttcttactctgtttcagacaactgttaatgcattctatgtttagtatatgtca
cttgagattaggctgacattttaccatttaaagcatcaaaatagaactattaaattgtttcatgaatagtggcaaggaagattttaaactggagagtagg
gcagccatacattttaagggcaaatgaaactgcctgtgcctgcactttcagggttattttatttcttcctataccatcagtaaatatgccacggattacc
caagttaaataactgtacaggctttctcactgcaatgttaataatatatgaggaatgccatctgctttactaaaagatggaggctgtttaatatacagtg
tttttgtagaaggtttgcaacataactaaaatgttaaacattttacaataaggtcatggtgttctatattgatttatattgtcaacaaaaaaatacctct
ggtcctcttgtatacagcaccatgtttttcttcctgttacagctggtatttctcttaaggcgctaacttggaacactggaatttaattattttattaaag
acagccccctgacacactcctttggggcactcatttctctcccttatcctaaagttaatcataaaactcattaaggaaagacccagattttgaaatatgt
aatgttttcattttttttccaagcttanacaaaagttgtctaaggctttgtgga
3' EST: DT393058 [+]atgtagaaattcacccttttaatccacaaagccttagacaacttttgtttaagctttggaaaaaaatgaaaacattacaatattcaaaatctgggtcttt
ccttaatgagttttatgattaactttaggataagggagagaaatgagtgccccaaaggagtgtgtcagggggctgtctttaataaaataattaaattcca
gtgttccaagttagcgccttaagagaaataccagctgtaacaggaagaaaaacatggtgctgtatacaagaggaccagaggtatttttttgttgacaata
taaatcaatatagaacaccatgaccttattgtaaaatgtttaacattttagttatgttgcaaaccttctacaaaaacactgtatattaaacagcctccat
cttttagtaaagcagatggcattcctcatatattattaacattgcagtgagaaagcctgtacagttatttaacttgggtaatccgtggcatatttactga
tggtataggaagaaataaaataaccctgaaagtgcaggcacaggcagtttcatttgcccttaaaatgtatggctgccctactctccagtttaaaatcttc
cttgccactattcatgaaacaatttaatagttctattttgatgctttaaatggtaaaatgtcagcctaatctcaagtgacatatactaaacatagaatgc
attaacagttgtctgaaacagagtaagaaaaaaaatttacatggcagcaaactgaagtgacaactaggaatgtggagaaacctgcgcttttctcatcgaa
cgcagagccttctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: