XB-CLONE-3182094
Clone Name: IMAGE:7852751
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Gene:
mien1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122005 [+]
AAAATGTCCGTCAGTATCGTGGTGGAGTATTGCGAGCCCTGCGGGTTCAAGTCTCACTATGAAGAGTTGGCAAGCGCCGTGCTTGAAGAATTTCCAGACG
TCACCATTGACTCCCGCCCTGGGGGCACAGGTGCCTTTGAGATTGAGATAAACGGACAGCTGGTTTTCTCCAAGCTGGAACTGGGGGGGTTTCCCTATGC
GAAAGATCTCATTGAGGCCATCCGAAAAGCCAGCAATGGGGAGCCAGTGGAGAAGATCACCAATAGTCAGGCCCCGTGTGTTATTTTATAACTCTCCAGG
TGCCACCTTTCTACCATGACTGCTGTAATGAATTGAATGACTTAGCGCTGCCTTTATAACAGTAGCATTGGTTCCTTTAGGTCTTGACCATTGTGTCCTC
ACCTGTAAGTGAAACCTGTTGGTATGTTCCAGCCATTAATAGCCATAAGGAAACAATTATACTCTAACGCTGAAAGCAAAAAATGGGAACACACTGGAAA
AGCCACAATGGTGCTTAGGATTGACAGTAACTACCATTTCTCAACTGCCTGTCCCCTACATACACATCATTCCCTCTTCTGTCAAAGGTTTGTGTCACAG
CCATACATATTGTCCATATATCCATATGCCTAAAGCAGCTTGGAATTAACCCATGTCCATAGTAGGCCAGGATTATGTACTTAATTATGTAATTAATTGC
TTAAATGCAGATGAGCTAAAGGGAATTTTGGCTCACTTTTCTATTTATTGGGGCATACGTAATCCTTGTGCTTTTTCCAGAGTGGGGGAATTTACACCAG
ATATAGATTACTATTAAAGGGGAAGTCCACCCAAACACAAGCAACTTTGCAATATAATATCAATTAAAAAATATGCAGCCTTTTCATGATTAATAATATG
GTTTGGAACAGTTGCCTAAGCCCGGCCCTCTATTCTCCTGCTGATCTGGCTGACTACTTTGAGACTCTTCCAGCACAAATTAGAATTTGGCTTCATTGTT
TGCCTCAATACCCAAAGCCCTGGCAGCAATCGGGGTACAACTCGAAGACCAATTACTGTGGTTTAGTATGTATGGGACAGGGGGCCTAATGAAGGAAGTG
GGTAGCTATTAGCAGCCCAGAATTCAGTATTTGTCTCCAAGTTATGTGTGTTAGGTCTAATATTACATCCAGATATAAACACTAATACTGTGTGGCTAAT
ATTGACTCACTTCTCATATTGGCATAATGGAGCACTTCAGGCTTCAGGGTAACCTCTCCTGATACTGACAGGCAAAGGATTATTACATTTTACAATAATG
ACACCTGTATTTATTTAAAAAGTGTAAATAATGCATTAAAATGTTCAGTTCTAAAAAAAAAAAAAAAAAA
5' EST: DT393081 [+]aaaatgtccgtcagtatcgtggtggagtattgcgagccctgcgggttcaagtctcactatgaagagttggcaagcgccgtgcttgaagaatttccagacg
tcaccattgactcccgccctgggggcacaggtgcctttgagattgagataaacggacagctggttttctccaagctggaactgggggggtttccctatgc
gaaagatctcattgaggccatccgaaaagccagcaatggggagccagtggagaagatcaccaatagtcaggccccgtgtgttattttataactctccagg
tgccacctttctaccatgactgctgtaatgaattgaatgacttagcgctgcctttataacagtagcattggttcctttaggtcttgaccattgtgtcctc
acctgtaagtgaaacctgttggtatgttccagccattaatagccataaggaaacaattatactctaacgctgaaagcaaaaaatgggaacacactggaaa
agccacaatggtgcttaggattgacagtaactaccatttctcaactgcctgtcccctacatacacatcattccctcttctgtcaaaggtttgtgtcacag
ccatacatattgtccatatatccatatgcctaaagcagcttggaattaacccatgtccatagtaggccaggattatgtacttaattatgtaattaattgc
ttaaatgcagatgagctaaagggaattttggctcacttttctatttattggggcatacgtaatccttgtgctttttccagagtgggggaatttacaccag
atatagattacta
3' EST: DT393080 [+]agaactgaacattttaatgcattatttacacctttttaaataaatacaggtgtcattattgtaaaatgtaataatcctttgcctgtcagtatcaggagag
gttaccctgaagcctgaagtgctccattatgccaatatgagaagtgagtcaatattagccacacagtattagtgtttatatctggatgtaatattagacc
taacacacataacttggagacaaatactgaattctgggctgctaatagctacccacttccttcattaggccccctgtcccatacatactaaaccacagta
attggtcttcgagttgtaccccgattgctgccagggctttgggtattgaggcaaacaatgaagccaaattctaatttgtgctggaagagtctcaaagtag
tcagccagatcagcaggagaatagagggccgggcttaggcaactgttccaaaccatattattaatcatgaaaaggctgcatattttttaattgatattat
attgcaaagttgcttgtgtttgggtggacttcccctttaatagtaatctatatctggtgtaaattcccccactctggaaaaagcacaaggattacgtatg
ccccaataaatagaaaagtgagccaaaattccctttagctcatctgcatttaagcaattaattacataattaagtacataatcctggcctactatggaca
tgggttaattccaagctgctttaggcatatggatatatggacaatatgtatggctgtgacacaaacctttgacagaagagggaatgatgtgtatgtaggg
gac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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